解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (2 ): 259-263.doi: 10.3969/j.issn.0529-1356.2013.02.022

• 组织学胚胎学发育生物学 • 上一篇    下一篇

zdhhc17对斑马鱼集中延伸运动的调节及其机制 

杨阳  高明  陈学冉  史玮  王芬  马保华  郝爱军   

  1. 山东大学医学院组织学与胚胎学教研室，教育部实验畸形学重点实验室， 济南250012
  • 收稿日期:2012-09-26 修回日期:2012-11-12 出版日期:2013-04-06 发布日期:2013-04-06
  • 通讯作者: 郝爱军 E-mail:aijunhao@sdu.edu.cn
  • 基金资助:

    教育部高等学校博士学科点专项科研基金资助项目;国家自然科学基金资助项目

Zdhhc17 regulating convergent and extension movements during zebrafish embryogenesis

YANG Yang  GAO Ming  CHEN Xue-ran  SHI Wei  WANG Fen  MA Bao-hua  HAO Ai-jun*   

  1. Key Laboratory of the Ministry of Education for Experimental Teratology, Department of Histology and Embryology, Shandong University School of Medicine, Ji’nan 250012, China
  • Received:2012-09-26 Revised:2012-11-12 Online:2013-04-06 Published:2013-04-06

摘要：

目的 通过抑制zdhhc17基因的表达，探讨其在斑马鱼胚胎发育过程中的作用及机制。方法 通过显微注射zdhhc17吗啉反义寡聚核苷酸(MO)抑制zdhhc17翻译，观察斑马鱼胚胎发育过程中相关表型的变化。利用斑马鱼整封原位杂交技术，检测集中延伸运动相关标记基因（dlx3b、ntl、myod1、 tbx6等）的表达变化。利用RT-PCR技术，检测集中延伸运动相关调控因子mRNA表达水平的变化。 结果 敲除zdhhc17基因后，斑马鱼胚胎集中延伸运动出现严重缺陷，主要表现为尾部弯曲变短（82/118），且集中延伸运动相关标记基因表达异常，钙黏附蛋白家族cdh1、padh18a mRNA水平降低。结论 zdhhc17通过调节钙黏附蛋白家族cdh1、padh18a基因的表达，进而影响细胞黏附运动，最终参与调控斑马鱼胚胎集中延伸运动。

关键词: zdhhc17 , 棕榈酰化 , 集中延伸运动 , 钙黏附蛋白家族 , 整封原位杂交 , 斑马鱼

Abstract:

Objective  To investigate the role of zdhhc17 on embryonic development in zebrafish by loss-of-function experiments. Methods Zdhhc17 antisense morpholino oligonucleotide was injected into embryos to block the translation. The whole mount in situ hybridyzation and RT-PCR were used to detect the abnormal development after knockdown of zdhhc17. Results Knockdown of zdhhc17 impacted convergence and extension movements during zebrafish gastrulation. The tail of zdhhc17 morphants was curved and shorter (82/118). The expression patterns of dlx3b, ntl, myod1 and tbx6 were affected. The mRNA level of cdh1 and padh18a was obviously lower in embryos injected with zdhhc17 morpholino. Conclusion zdhhc17 is critical for zebrafish convergence and extension movements via regulating the expression of cadherin superfamily genes cdh1 and pcdh18a and affecting cell adhesion.

Key words: zdhhc17 , Palmitoylation , Convergence and extension movements , Cadherin superfamily , Whole mount in situ hybridization , Zebrafish