pcgf5a在斑马鱼胚胎早期发育中的作用

doi:10.16098/j.issn.0529-1356.2023.01.015

解剖学报 ›› 2023, Vol. 54 ›› Issue (1): 99-103.doi: 10.16098/j.issn.0529-1356.2023.01.015

• 组织学胚胎学发育生物学 • 上一篇下一篇

pcgf5a在斑马鱼胚胎早期发育中的作用

周娟周文娟李心悦史玮郝爱军*

山东大学基础医学院组织学胚胎学系，教育部实验畸形学重点实验室，山东省精神疾病基础与临床重点实验室，济南 250012

收稿日期:2021-04-08 修回日期:2022-09-26 出版日期:2023-02-06 发布日期:2023-02-06
通讯作者: 郝爱军 E-mail:aijunhao@sdu.edu.cn
基金资助:
国家自然科学基金资助项目;山东大学荣祥再生医学基金项目

Role of pcgf5a in the early development of zebrafish

ZHOU Juan ZHOU Wen-juan LI Xin-yue SHI Wei HAO Ai-jun*

Key Laboratory for Experimental Teratology of Ministry of Education, Shandong Key Laboratory of Mental Disorders, Department of Anatomy and Histoembryology, School of Basic Medical Sciences, Shandong University, Ji’nan 250012, China

Received:2021-04-08 Revised:2022-09-26 Online:2023-02-06 Published:2023-02-06
Contact: HAO Ai-jun E-mail:aijunhao@sdu.edu.cn

摘要/Abstract

摘要：

目的探讨pcgf5a基因在斑马鱼胚胎发育过程中的作用和机制。方法通过显微注射pcgf5a吗啉代寡核苷酸（MO）抑制pcgf5a基因mRNA翻译过程，观察斑马鱼胚胎发育过程中相关表型的变化；利用胚胎整封原位杂交和Real-time PCR等技术检测抑制pcgf5a对胚胎神经及眼睛发育相关标记基因的表达变化。结果抑制pcgf5a基因后，斑马鱼胚胎中外胚层发育受到影响，表现为脑区变小，眼睛发育迟缓，尾部卷曲及体轴缩短（548/891），同时神经发育（sox2：82/98、sox3：73/84、foxg1：70/88、pax6a：36/45）和眼睛发育（pax2a、vsx2和rx1，n=5）相关基因表达明显降低。结论 pcgf5a可能通过调节神经与眼睛发育相关转录因子的表达，进而影响细胞增殖和凋亡而参与胚层分化以及后续的神经系统与眼睛的发育。

关键词: pcgf5a基因, 神经系统, 眼睛发育, 整封原位杂交, 实时定量聚合酶链反应, 斑马鱼

Abstract:

Objective To investigate the role of pcgf5a gene during zebrafish embryonic development. Methods pcgf5a morpholine antisense morpholino oligomers(MO) was microinjected to delete the expression of pcgf5a gene, and possible phenotypes were examined in pcgf5a-deficient embryos. The whole mount in situ hybridization and Real-time PCR were used to detect alterations of key genes related to the development of nervous system and eye. Results After pcgf5a gene knockdown, the development of ectoderm and mesoderm of zebrafish embryos were affected. The brain size became smaller, and the eyes developed retarded, and the tail was curled and the body axis was shortened（548/891）. In addition, the expressions of sox2(82/98), sox3（73/84）, foxg1（70/88）, pax6a（36/45）, pax2a, vsx2 and rx1(n=5) were significantly reduced. Conclusion pcgf5a affects the morphogenesis of nervous system and eye, possibly due to regulating the expression of the transcription factors related to their development via the processes of cell proliferation and apoptosis.

Key words: pcgf5a gene, Nervous system, Eye development, Whole mount in situ hybridization, Real-time PCR, Zebrafish

中图分类号:

R394.1

周娟周文娟李心悦史玮郝爱军. pcgf5a在斑马鱼胚胎早期发育中的作用[J]. 解剖学报, 2023, 54(1): 99-103.

ZHOU Juan ZHOU Wen-juan LI Xin-yue SHI Wei HAO Ai-jun. Role of pcgf5a in the early development of zebrafish[J]. Acta Anatomica Sinica, 2023, 54(1): 99-103.

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pcgf5a在斑马鱼胚胎早期发育中的作用

Role of pcgf5a in the early development of zebrafish

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