解剖学报 ›› 2014, Vol. 45 ›› Issue (3): 344-349.doi: 10.3969/j.issn.0529-1356.2014.03.009

• 肿瘤生物学 • 上一篇    下一篇

MT2受体介导褪黑素对小鼠胃癌细胞的抑制作用

 徐丽1,2金清东1 宫喜2 刘卉2 周瑞祥2*   

  1. 1. 莆田学院基础医学院生理学教研室,福建 莆田 351100; 2. 福建医科大学人体解剖学与组织学胚胎学系 神经生物学研究中心, 福州 350004
  • 收稿日期:2013-12-02 修回日期:2014-01-24 出版日期:2014-06-06 发布日期:2014-06-06
  • 通讯作者: 周瑞祥 E-mail:zhourx@mail.fjmu.edu.cn
  • 基金资助:

    国家自然科学基金资助;福建省科技厅重点项目;福建省自然科学基金资助;莆田市科技计划项目

Inhibitory effect of melatonin on murine foregastric carcinoma cells via membrane receptors MT2

XU Li 1,2 JIN Qing-dong1 GONG Xi2 LIU Hui2 ZHOU Rui-xiang 2*   

  1. 1. Department of Physiology, Basic Medical College of Putian University, Fujian Putian 351100, China; 2. Department of Human Anatomy and Histology and Embryology, Neurobiology Research Center, Fujian Medical University, Fuzhou 350004, China
  • Received:2013-12-02 Revised:2014-01-24 Online:2014-06-06 Published:2014-06-06
  • Contact: ZHOU Rui-xiang E-mail:zhourx@mail.fjmu.edu.cn

摘要:

目的 探讨褪黑素(MLT)通过褪黑素膜受体MT2抑制小鼠前胃癌(MFC)细胞增殖及其与丝裂原活化蛋白激酶(MAPKs)、磷脂酰肌醇-3激酶(PI3K)-Akt信号通路的关系。方法 应用siRNA技术沉默MT2表达,观察褪黑素对小鼠前胃癌细胞的抑制作用及ERK1/2、Akt的磷酸化的影响。结果 1.siRNA介导的MT2沉默能明显拮抗褪黑素对胃癌细胞增殖的抑制作用;2. 沉默MT2可部分阻断褪黑素抑制ERK1/2、Akt磷酸化的作用。结论 褪黑素可通过MT2受体抑制ERK1/2、Akt的磷酸化从而抑制胃癌细胞增殖。

关键词: 褪黑素, 小鼠前胃癌细胞, 褪黑素膜受体MT2, Akt, ERK1/2, 实时定量聚合酶链反应, 小鼠

Abstract:

Objective To investigate the inhibitory effect of melatonin on the proliferation activity of murine foregastic carcinomac (MFC) cells via melatonin membrane receptors MT2 and its relationship with the signaling pathways of mitogen-activated protein kinases (MAPKs), phosphatidylinositol 3-kinase(PI3K)-Akt. Methods Using siRNA technology to silence MT2 expression, we examined the ability of melatonin to inhibit the proliferation activity of MFC cells and its influence on the phosphorylation of ERK1/2 and Akt. Results We found two interesting effects of SiRNA-mediated silencing of MT2 expression. Firstly, it significantly antagonized the inhibitory effect of melatonin on the proliferation activity of MFC cells. Secondly, it partially blocked the inhibitory effect of melatonin on the phosphorylation of ERK1/2 and Akt. Conclusion Our results suggest that melatonin can inhibit the phosphorylation of ERK1/2 and Akt via MT2 receptors, thereby inhibiting the proliferation of gastric cancer cells.

Key words: Melatonin, Murine foregastic carcinoma cell, Melatonin membrane receptor MT2, Akt, ERK1/2, Real-time PCR, Mouse