解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (4): 465-468.doi: 10.3969/j.issn.0529-1356.2014.04.005

• 神经生物学 • 上一篇    下一篇

孕激素受体在雌性山羊颈胸神经节的表达

郭晓 李强 陈文东 徐永平* 金秀芳 王志豪 董伟 范洁   

  1. 西北农林科技大学动物医学院基础解剖学研究室，陕西 杨凌 712100
  • 收稿日期:2013-05-07 修回日期:2013-07-15 出版日期:2014-08-06 发布日期:2014-08-06
  • 通讯作者: 徐永平 E-mail:xuyp717@nwsuaf.edu.cn
  • 基金资助:

    国家自然科学基金项目; 陕西省农业推广项目

Expression of progesterone receptor in the cervicothoracic ganglion of female goat

GUO Xiao  LI Qiang  CHEN Weng-dong  XU Yong-ping*  JIN Xiu-fang  WANG Zhi-hao  DONG Wei  FAN Jie   

  1. Basic Anatomy Laboratory, College of Veterinary Medicine, Northwest A﹠F University, Shanxi Yangling 712100，China
  • Received:2013-05-07 Revised:2013-07-15 Online:2014-08-06 Published:2014-08-06
  • Contact: XU Yong-ping E-mail:xuyp717@nwsuaf.edu.cn

摘要：

目的 观察雌性山羊颈胸神经节内神经元是否具备接受孕激素作用的条件。方法 采用免疫组织化学SP法观察孕激素受体(PR)在成年雌性山羊颈胸神经节的表达。结果 雌性山羊颈胸神经节内神经元胞体均呈孕激素受体免疫反应阳性；卫星细胞、施万细胞、神经突起和神经纤维中也有较弱的着色。神经元的细胞膜和细胞质呈棕褐色为强阳性，而神经元的细胞核着色出现异质性：85.10%的神经元细胞核为强阳性，其中31.91%的核仁呈清亮的空泡，为阴性；而14.90%的神经元细胞核呈空泡状不着色，其中7-45%的核仁为强阳性。卫星细胞、施万细胞和神经纤维均呈淡黄色，为弱阳性。图像分析表明，神经元胞体PR的相对表达量与其他非神经成分相比差异性极显著（P＜0.01）。 结论 在颈胸神经节内神经元是孕激素作用的主要靶细胞之一，提示孕激素有可能通过影响雌性山羊颈胸神经节内神经元的活动参与心脏功能的神经调节，而颈胸神经节上的PR可能作为一个网络节点协调孕激素对心脏功能的内分泌调节和自主神经对心脏活动的神经调节。

关键词: 颈胸神经节, 孕激素受体, 免疫组织化学SP法, 雌山羊

Abstract:

Objective  In order to explore whether neurons in the cervicothoracic ganglion of female goat were equipped with the condition for the role of progesterone.  Methods Immunohistochemical SP method was used to detect the expression of progesteron receptor(PR) in cervicothoracic ganglion of female goats. Results The result indicated that PR-immunoreactive-products were mainly present in the cell bodies of neurons,and satellite cells, Schwann cells, neurapophysis and nerve fiber also had weaker staining.The cell membrane and cytoplasm of neurons represented brown as strong positive,but the nucleus of neurons showed the presence of heterogeneity, 85.10% neurons’ nucleus was strong positive,of which 31.91% nucleolus being clear vacuoles were negative; 14.90% nucleus displaying vacuolization had no shaining, of which 7.45% nucleolus was strong positive. There were weak positive and flaxen PR-immunoreactive-products in satellite cells, Schwann cells, neurapophysis and nerve fiber. Image analysis shows that PR of neurons was extremely significant compared with other nonnerve cells（P＜0.01). Conclusion The result proved that the sympathetic postganglionic neurons in cervicothoracic ganglion of female goat were the one of main target cells of progesterone,suggesting that progesterone may be involving in the neuroregulation of cardiac functional activities through affectinng the activities of neurons in the cervicothoracic ganglion of female goat,and PR in the cervicothoracic ganglion may act as a network node to coordinate endocrine regulation of progesterone and neuroregulation of autonomic nerve on the cardiac functional activities.

Key words: Cervicothoracic ganglion, Progesterone receptor, Immunohistochemical SP method, Female goat