解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (2 ): 157-162.doi: 10.3969/j.issn.0529-1356.2013.02.003

• 神经生物学 • 上一篇    下一篇

孕期尼古丁暴露致成年子代大鼠中枢对血管紧张素II的高反应性

俞峰1 张建国1 高喜仁1 董伟家1 张志智1 张博1 夏志恒1 张于2* ..   

  1. 1.湖州师范学院医学院，浙江 湖州 313000; 2.苏州大学医学部实验中心，江苏 苏州 215123
  • 收稿日期:2012-06-25 修回日期:2012-08-23 出版日期:2013-04-06 发布日期:2013-04-06
  • 通讯作者: 张于娟 E-mail:yufeng4205@sina.com
  • 基金资助:

    市科技项目;其他(不属于以上基金类别的请自行输入下框)

Prenatal nicotine exposure induces adult offspring high response to central angiotensin II

YU Feng1 ZHANG Jian-guo1 GAO Xi-ren1 DONG Wei-jia1 ZHANG Zhi-zhi1 ZHANG Bo1 XIA Zhi-heng1 ZHANG Yu-juan 2*   

  1. 1.Medical Department of Huzhou Teacher’s College,Zhejiang Huzhou 313000,China; 2. Experiment Center, Medicial College, Suzhou University, Jiangsu Suzhou 215123, China
  • Received:2012-06-25 Revised:2012-08-23 Online:2013-04-06 Published:2013-04-06

摘要：

目的 探讨孕期尼古丁暴露后，子代大鼠中枢对血管紧张素II（AngII）的反应性。方法 对孕期尼古丁暴露的子代大鼠（n =7）脑室内注射AngII、洛沙坦(Los)和PD123319（PD）后，观察尼古丁子代（尼古丁组）平均动脉压（MAP）、血气与正常大鼠子代（对照组，n =7）之间的差异，并检测下丘脑前区（AHA）c-Fos表达，AngII受体1a（AT1aR）、AT1bR和AT2R mRNA的变化，以及AHA区AT1R、AT2R蛋白表达水平。结果 尼古丁组基础MAP与对照组无差异，但脑室内注射 AngII后，尼古丁组大鼠MAP高于对照组［（120.36±6.23） mmHg （109.87±6.86）mmHg，P <0.05］，AHA区的c-Fos表达也明显强于对照组（25.8±2.91 比6.42±1.52，P <0.05），而Los预处理后，两组MAP无明显变化，但PD预处理后，尼古丁组MAP仍高于对照组。并且尼古丁组AHA区的AT1aR mRNA高于对照组（1.23±0.05 比 1.00， P <0.05），AT1R蛋白表达也高于对照组（0.581±0.06 比 0.353±0.05，P <0.05）。结论 孕期尼古丁暴露可导致子代大鼠AHA区AT1aR mRNA及AT1R蛋白高于对照组，可能导致中枢对AngII的反应性增强。

关键词: 孕期 , 尼古丁, 下丘脑前区 , 高血压 , 脑室插管 , 免疫组织化学 , 大鼠

Abstract:

Objective To investigate the effect of prenatal nicotine exposure to the adult offspring response to central angiotensin. Methods The mean arterial pressure(MAP) and blood gas analysis after intracerebroventricular (ICV)injection of angiotensin II(AngII), losartan(Los), PD123319（PD）to nicotine offspring （nicotine, n =7）and control offspring（control, n =7） were tested. The angiotensin Ⅱ receptor 1 (AT1aR), AT1bR and AT2R mRNA, c-Fos protein, and AT1R, AT2R protein expression of anterior hypothalamic area(AHA) were detected. Results There was no difference between the control and nicotine groups, but after ICV AngII, the MAP was significantly higher in nicotine group than in the control group ［（120.36±6.23）mmHg vs （109.87±6.86）mmHg，P <0.05］. ICV Los in advance blocked the effect whereas ICV PD did not. The c-Fos expression was stronger in the nicotine group than that in the control group（25.8±2.91 vs 6.42±1.52, P< 0.05）. The AT1aR mRNA（1.23±0.05 vs 1.00，P < 0.05）and AT1R protein（0.581±0.06 vs 0.353±0.05，P <0.05）expression were higher in the nicotine group than that in the control group. Conclusion Prenatal nicotine exposure induces higher expression of AT1aR mRNA and AT1R protein, which may result in high response to central angiotensin II of adult offspring.

Key words: Pregnancy , Nicotine , Anterior hypothalamic area , Hyperthension, Cerebral cannula , Immunohistochemistry , Rat