解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (5 ): 602-606.doi: 10.3969/j.issn.0529-1356.2013.05.003

• 神经生物学 • 上一篇    下一篇

腹腔注射2-脱氧-D-葡萄糖可诱发大鼠视上核和室旁核神经元表达Fos

赵琳1  贾鲲鹏2  金晓航3  史娟3*   

  1. 1. 延安大学医学院人体解剖学教研室，陕西 延安 716000; 2. 延安大学附属医院儿科，陕西 延安 716000; 3. 第四军医大学人体解剖学与组织学胚胎学教研室，西安 710032
  • 收稿日期:2012-10-10 修回日期:2013-01-11 出版日期:2013-10-06 发布日期:2013-10-06
  • 通讯作者: 赵琳 E-mail:jkpzhaolin@163.com
  • 基金资助:

    国家自然科学基金重点资助项目

Intraperitoneal injection of 2-DG induces Fos expression in the hypothalamus supraoptic nucleus and paraventricular nucleus of rats

ZHAO Lin1 JIA Kun-peng2 JIN Xiao-hang3 SHI Juan 3*   

  1. 1. Department of Human Anatomy, Medical College of Yan’an University, Shaanxi Yan’an 16000,China;2. Department of Pediatrics, Affiliated Hospital of Yan’an University, Shaanxi Yan’an 716000,China; 3. Department of Human Anatomy,Histology and Embryology, the Fourth Military Medical University, Xi’an 710032, China
  • Received:2012-10-10 Revised:2013-01-11 Online:2013-10-06 Published:2013-10-06

摘要：

目的 探讨腹腔注射2-脱氧-D-葡萄糖（2-DG）能否激活大鼠下丘脑视上核（SON）和室旁核（PVN）神经元而表达Fos。方法 健康雄性SD大鼠12只，随机分为腹腔注射2-DG组（6只）、生理盐水对照组（3只）及正常对照组（3只）。各自处理后，应用免疫组织化学方法，观察各组下丘脑SON和PVN内Fos表达及其与催产素（OT）和加压素（VP）的双标情况，同时采用ELISA方法对血清中OT和VP的含量进行检测。 结果与生理盐水对照组和正常对照组相比，2-DG引发的特异性Fos免疫阳性产物主要集中分布于下丘脑外侧区和穹隆周区，在SON、PVN也有密集表达。SON和PVN内的Fos表达与该区的特异性神经活性物质OT和VP有共存。OT/Fos双标细胞率（双标细胞占OT阳性细胞的百分率）在SON和PVN分别为87.10%、90.57%，明显高于VP/Fos在这两个核团的双标率（双标细胞占VP阳性细胞的百分率，68.42%、76.92%），两者比较差异有统计学意义（P<0.05）。ELISA检测结果显示，2-DG组动物血清中OT和VP水平与对照组相比无明显变化。 结论 腹腔注射2-DG可激活大鼠下丘脑SON和PVN内OT和VP神经元表达Fos，SON和PVN可能参与2-DG诱导的急性应激反应。

关键词: 2-脱氧-D-葡萄糖, 视上核, 室旁核, Fos, 催产素, 加压素, 免疫组织化学, 酶联免疫吸附测定

Abstract:

Objective To study if intraperitoneal injection of 2-deoxy-D-glucose (2-DG) can functionally activate neurons in the hypothalamus supraoptic nucleus (SON) and paraventricular nucleus (PVN) by induction of Fos expressing.
Methods Twelve healthy male Sprague-Dawley rats were randomly divided into intraperitoneal injection of 2-DG (6 rats), saline control (3 rats) and normal control (3 rats) groups. After the treatment, immunohistochemical staining was employed to observe the expressions of Fos and double labelling of oxytocin (OT) or vasopressin (VP) with Fos in SON and PVN, respectively. The plasmal level of OT and VP was examined by ELISA assay. Results Compared with saline and normal control groups, 2-DG induced specific Fos expression was mainly located in lateral and perifornical areas of hypothalamus. High levels of Fos immunoreactivity were observed in SON and PVN where they double labeled with the specific neuroactive substances OT and VP. The double labeling rates for OT and Fos (the percentage between the number of OT/Fos doublelabeled neurons to that of OT positive neurons) were 87.10% in SON and 90.57% in PVN, significantly higher than that of VP/Fos double labeling rate (68.42% in SON, 76.92% in PVN, P<0.05). In contrast, the plasma level of OT and VP after injection of 2-DG may not show discernible changes to that of control rat. Conclusion Intraperitoneal injection of 2-DG can induce Fos expression in OT and VP neurons in hypothalamic SON and PVN which may be involved in triggering acute stress response induced by 2-DG.

Key words: 2-Deoxy-D-glucose, Supraoptic nucleus, Paraventricular nucleus, Fos, Oxytocin, Vasopressin, Immunohistochemical, ELISA

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