解剖学报

›› 2012, Vol. 43 ›› Issue (5): 690-693.doi: 10.3969/j.issn.0529-1356.2012.05.020

• 组织学胚胎学发育生物学 • 上一篇    下一篇

JNK/c-Jun信号通路在大鼠胸主动脉瘤模型中的表达及意义

范军1; 柏树令1* ; 李想2; 阎亚伟1; 田晓红1; 侯伟健1; 佟浩1   

  1. 1.中国医科大学基础医学院组织工程学教研室; 2.中国医科大学基础医学院细胞生物学教研室，沈阳 110001
  • 收稿日期:2011-11-10 修回日期:2012-01-16 出版日期:2012-10-06
  • 通讯作者: 柏树令

Expression and significance of JNK/c-Jun pathway in rat thoracic aortic aneurysm

  1. 1.Department of Tissue Engineering, College of Basic Medical Sciences, China Medical University, Shenyang 110001，China; 2.Department of Cell Biology, College of Basic Medical Sciences, China Medical University, Shenyang 110001，China
  • Received:2011-11-10 Revised:2012-01-16 Online:2012-10-06
  • Contact: BAI Shu-ling

关键词: c-Jun氨基末端激酶, c-Jun, 胸主动脉瘤, 免疫组织化学, 免疫印迹法, 大鼠

Abstract: Objective To investigate the expression and significance of c-Jun N-terminal kinase (JNK) and c-Jun in the experimental rat thoracic aortic aneurysm. BR>Methods Twenty adult male Wistar rats were randomly divided into two groups: sham group and operated group. The thoracic aortic aneurysm models were induced by CaCl2 The thoracic aortic segments were collected at the 4th week after surgery. Immunohistochemistry and Western blotting were performed to detect the expression of JNK and c-Jun in the wall of the aneurysm. Results Immunohistochemical staining results showed that p-JNK and p-c-Jun were located extensively in smooth muscle cells of the tunica media in the aortic aneurysm, while there was only a little positive staining in the control group. Western blotting results indicated that the expression levels of p-JNK and p-c-Jun in the aortic aneurysm were higher than those in the control. Conclusion The expressions of p-JNK and p-c-Jun in thoracic aortic aneurysm are stronger than that in the control. The JNK/c-Jun pathway may play a significant role in the development of aortic aneurysm. BR>

Key words: c-Jun N-terminal kinase, c-Jun, Thoracic aortic aneurysm, Immunohistochemistry, Western blotting, Rat

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