解剖学报

›› 2012, Vol. 43 ›› Issue (6): 813-817.doi: 10.3969/j.issn.0529-1356.2012.06.018

• 组织学胚胎学发育生物学 • 上一篇    下一篇

海洛因戒断复吸对大鼠睾丸表皮生长因子、嗜铬粒素A表达及血清卵泡刺激素与黄体生成素的影响

谢莉; 梁文妹*   

  1. 贵阳医学院组织学胚胎学教研室，贵阳 550004
  • 收稿日期:2011-12-07 修回日期:2012-01-16 出版日期:2012-12-06
  • 通讯作者: 梁文妹

Influence of heroin withdrawal and relapse on testicular epidermal growth factor, chromogranin A immunoreactive cells and serum FSH,LH level in rats

  1. Department of Histology and Embryology, Guiyang Medical College, Guiyang 550004, China
  • Received:2011-12-07 Revised:2012-01-16 Online:2012-12-06
  • Contact: LIANG Wen-mei

摘要： 目的 探讨海洛因戒断、复吸对大鼠睾丸表皮生长因子(EGF)、嗜铬粒素A(CgA)免疫反应（IR）细胞、血清卵泡刺激素（FSH）与黄体生成素（LH）的影响。方法 正常雄性SD大鼠63只，随机分为实验组21只、盐水对照组21只和正常对照组21只，实验组大鼠又分戒断组、脱毒组和复吸组。取正常对照组、盐水对照组及实验各组大鼠的睾丸组织和血清，采用免疫组织化学SABC法、图像分析法及放射免疫法进行研究。 结果 戒断组中EGF-IR细胞染色加深，免疫反应强度增强，平均灰度值降低（ EM>P /EM><0.05）；脱毒组EGF-IR细胞免疫反应强度有所下降，但仍强于正常及盐水对照组（ EM>P /EM><0.05）；复吸组EGF-IR细胞免疫反应强度减弱，平均灰度值升高（ EM>P /EM><0.05）。戒断组CgA-IR细胞免疫反应强度减弱

关键词: 海洛因戒断, 海洛因复吸, 表皮生长因子, 嗜铬粒素A, 睾丸, 免疫组织化学, 放射免疫测定, 大鼠

Abstract: Objective To study the changes of testicular epidermal growth factor (EGF), chromogranin A(CgA)immunoreactive(IR) cells, and the changes of serum follicle-stimulating hormone（FSH）, luteinizing hormone（LH） level in rats during heroin withdrawal, methadone detoxification and heroin relapse, in order to investigate the mechanisms. Methods Sixty-three adult male SD rats were randomly divided into normal control group (NCG), saline control group (SCG),and experiment group (EG). The rats in the EG group were divided into heroin withdrawal group (HWG), methadone detoxication group (MDG) and heroin relapse group (HRG). Serum was obtained via heart puncture, and the testis was excised soon after heart puncture. Immunohistochemical SABC method, image analysis, and radioimmunoassay were used. Results Compared with NCG and SCG, the mean gray value of EGF-IR cells drawdown in HWG, and revived after the treatment of methadone but still at a high secretion state. In HRG the mean gray value of EGF-IR cells was increased. Compared with NCG and SCG, the mean gray value of CgA-IR cells was declined in HWG, which restored after methadone disposal. In HRG, the immune staining intensity of CgA-IR cells declined. Serum LH levels in HWG and HRG were at a low level, but FSH had no statistically significant in the whole process. Conclusion The testis secretion of EGF,CgA and serum LH level was changed during heroin withdrawal and relapse. The results suggest that the testis secretion of EGF,CgA and serum LH may participate in the process of body adjusting during heroin withdrawal, methadone detoxication and heroin relapse per

Key words: Heroin withdrawal, Heroin relapse, Epidermal growth factor, chromogranin A, Testis, Immunohistochemistry, Radioimmunoassay, Rat

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