解剖学报

›› 2007, Vol. 38 ›› Issue (4): 405-409.doi:

• 论著 • 上一篇    下一篇

突触后密度蛋白-93在大鼠脊髓损伤后的表达变化

高尚锋1,2; 程纯2; 陈梦玲2 ;赵剑3; 李欣2;牛淑琼1 ;严斌3 ;沈爱国1*   

  1. 1南通大学江苏省神经再生重点实验室，南通 226001;2南通大学基础医学院微生物与免疫学教研室，南通 226001;3南通大学附属医院骨科，南通 226001
  • 收稿日期:2006-10-09 修回日期:2007-01-11 出版日期:2007-08-06
  • 通讯作者: 沈爱国

EXPRESSION OF POSTSYNAPTIC DENSITY PROTEIN-93 IN INJURIED SPINAL CORD OF RAT

  1. 1The Jiangsu Province Key Laboratory of Neural Regeneration, Nantong University, Nantong 226001;2Department of Microbiology and Immunology, Basical Medical College, Nantong University, Nantong 226001;3Department of Orthopaedics, the Affiliated Hospital of Nantong University, Nantong 226001, China
  • Received:2006-10-09 Revised:2007-01-11 Online:2007-08-06
  • Contact: SHEN Ai-guo

关键词: 突触后密度蛋白-93, 脊髓损伤, 神经型一氧化氮合酶, 实时定量PCR, 免疫印迹法, 免疫荧光, 大鼠

Abstract: Objective To study the expression and distribution of postsynaptic density protein-93 (PSD-93) after spinal cord injury (SCI). Methods With improved Allen’s method, acute rats SCI models were established. Realtime PCR and Western blotting were used to detect the changes of PSD-93 mRNA and protein expression after SCI. The changes of PSD-93 localization after injury were investigated by immunofluorescence double staining. Results The expression of PSD-93 mRNA had a gradually decrease trend after SCI. The minimal mRNA was present at 3days; whereas the protein levels upregulated significantly and peaked at 1day after injury. Immunofluorescence double staining indictated that PSD-93 was highly expressed on the activated microglias and astrocytes except that it colocalized with nNOS in the impaired spinal cord.Conclusion The time course of PSD-93 and its mRNA is vary significantly after SCI, and colocalized with nNOS and activated glias. These results indicate that PSD-93 may participate in the f

Key words: PSD-93, Spinal cord injury, nNOS, Realtime PCR, Western blotting, Immunofluorescence, Rat

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