解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (1 ): 68-72.doi: 10.3969/j.issn.0529-1356.2013.01.01.013

• 细胞和分子生物学 • 上一篇    下一篇

脑红蛋白在成年牦牛不同组织细胞中的分布

曹亮1  刘霞2  石宁宁2  李盛杰2  杜晓华1*   

  1. 1.甘肃农业大学动物医学院; 2.生命科学技术学院，兰州 730070
  • 收稿日期:2012-06-21 修回日期:2012-07-29 出版日期:2013-02-06 发布日期:2013-02-06
  • 通讯作者: 杜晓华 E-mail:duxh@gsau.edu.cn
  • 基金资助:

    省自然科学基金资助项目

Distribution of neuroglobin in different tissues and cells of the adult yak

CAO Liang1 LIU Xia2 SHI Ning-ning2 LI Sheng-jie2 DU Xiao-hua1*   

  1. 1.College of Veterinary Medicine; 2.College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2012-06-21 Revised:2012-07-29 Online:2013-02-06 Published:2013-02-06

摘要：

目的 探讨脑红蛋白（NGB）在成年牦牛不同组织细胞中的分布特征。方法 选择健康成年牦牛8只，利用免疫组织化学染色SP法，观察NGB在成年牦牛不同组织细胞中的分布特征。结果 在成年牦牛体内，NGB主要表达于神经细胞，肾上腺、腺垂体及胰岛组织细胞，雄性生殖系统睾丸、睾丸输出小管及附睾管上皮细胞。NGB免疫阳性物质定位于细胞质。本研究在哺乳动物消化系统皱胃胃底腺分泌细胞及十二指肠腺分泌细胞中发现有NGB阳性表达。结论 在牦牛神经系统、内分泌系统、生殖系统以及消化系统组织细胞中有高表达，提示NGB在这些组织的氧利用及功能活动中发挥作用。

关键词: 脑红蛋白, 分布, 免疫组织化学, 牦牛

Abstract:

Objective To explore the localization of neuroglobin (NGB) in adult yak tissues and cells. Methods Eight health adult yaks were used in this study. NGB expression in adult yak tissues and cells was localized using the immunohistochemical SP technique. Results NGB-positive cells were mainly distributed in nerve cells, adrenal gland, adenohypophysis and pancreas islet, testis, efferent ductules of testis and epididymal epithelium of genital system. NGB-immunoreactive product was located in the cytoplasm. The NGB-positive cells were found in the fundic glands of abomasum and duodenal glands.Conclusion The high expression of NGB in tissues and cells of nervous system, endocrine system, genital system and alimentary system in yak suggested that NGB might play an important role in the utilization of oxygen and physiological functions.

Key words: Neuroglobin, Distribution, Immunohistochemistry, Yak