解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (2 ): 235-237.doi: 10.3969/j.issn.0529-1356.2013.02.017

• 肿瘤生物学 • 上一篇    下一篇

新疆哈萨克族食管癌中Survivin的表达及其与半胱氨酸蛋白caspase-9、caspase-3基因的相关性

李秀梅1  陈艳2  李卉3  庞作良4  王洪江4  姜孝芳3  郭琼5  李惠武3*    

  1. 1. 新疆医科大学形态学中心; 2. 生物化学教研室; 3. 科研中心; 4. 肿瘤附属医院; 5. 组织学胚胎学教研室，乌鲁木齐 830011
  • 收稿日期:2011-08-12 修回日期:2012-11-14 出版日期:2013-04-06 发布日期:2013-04-06
  • 通讯作者: 李秀梅 E-mail:dr.lxm@163.com
  • 基金资助:

    国家自然科学基金资助项目

Expression survivin and correlation with Caspase-9 and Caspase-3 genes in esophageal cancer of Kazakin in Xinjiang

LI Xiu-mei1  CHEN Yan2  LI Hui3  PANG Zuo-liang4  WANG Hong-jiang4 JIANG Xiao-fang3   GUO Qiong5  LI Hui-wu 3*    

  1. 1. Morphology Centre; 2. Department of Biochemistry; 3. Scientific Research Centre; 4. the Affiliated Toumour Hospital;5. Department of Histology and Embryology, Xinjiang Medical University, Urumqi 830011, China
  • Received:2011-08-12 Revised:2012-11-14 Online:2013-04-06 Published:2013-04-06

摘要：

目的 探讨新疆哈萨克族食管癌中Survivin基因表达及其与半胱氨酸蛋白Caspase-9、Caspase-3基因的关系。方法 首先从52例哈萨克族食管癌标本中筛选出34例Survivin mRNA差异表达的标本（癌组织中的表达量高于远端无癌组织），采用RT-PCR、Western blotting方法检测半胱氨酸蛋白Caspase-9、Caspase-3 mRNA和蛋白的表达。结果 1. Survivin在新疆哈萨克族食管癌组织高表达，差异具有统计学意义（P <0.05）；2. 在Survivin差异表达的34例标本中, 61.8%的肿瘤组织半胱氨酸蛋白Caspase-9低表达，并且 Survivin mRNA与Caspase-9 mRNA的表达呈负相关（r = -0.244，P <0.05）；3. 哈萨克族食管癌组织中，Caspase-9的蛋白活化降低（P <0.05）。结论 Survivin表达上调在新疆哈萨克族食管癌的发生、发展过程中发挥重要作用，Survivin可能通过直接抑制Caspase-9的转录以及抑制其活化水平发挥调控细胞凋亡的作用。

关键词: 食管癌 , Survivin , Caspase-3 , Caspase-9 , 反转录-聚合酶链反应 , 免疫印迹法 ,  哈萨克族

Abstract:

Objective To investigate the high expressed Survivin and its relationship with the apoptosis proteins in esophageal cancer of Kazakh in Xinjiang. Methods Thirty-four Survivin mRNA differential expression specimens from 52 cases of Kazakh’s esophageal cancer specimen (cancerous tissue expression in quantity than distal normal tissue) were screened. Caspase-9 and Caspase-3 gene and protein expression were detected by RT-PCR and Westernblotting respectively. Results 1. Survivin overexpressed in Kazakh’s esophageal cancer tissue, compared with distal normal tissue, differences were statistically significant (P <0.05); 2. 61.8% of the specimens had Caspase-9 low expression in 34 case specimens with Survivin mRNA differential expression, and the expression of Survivin was negatively correlated to the Caspase-9 expression（r = -0.244，P <0.05）; 3. Caspase-9 protein activation significantly decreased（P <0.05）. Conclusion The overexpression of Survivin may play an important role in the origin and development of Xinjiang Kazakh’sesophageal cancer. The function of Survivin in regulation of apoptosis may be related to direct inhibition of Caspase-9 and activation of transcription.

Key words: Esophageal cancer , Survivin , Caspase-3 , Caspase-9 , RT-PCR , Western blotting , Kazakin nationality