解剖学报 ›› 2014, Vol. 45 ›› Issue (4): 516-520.doi: 10.3969/j.issn.0529-1356.2014.04.013

• 肿瘤生物学 • 上一篇    下一篇

NonO蛋白在丁酸钠诱导小鼠红白血病细胞分化过程中的表达

桑婷婷 胡江江 薛建有 戚武林 赵辅昆 张世馥*   

  1. 浙江理工大学 生命科学学院, 杭州 310018
  • 收稿日期:2013-12-13 修回日期:2014-03-16 出版日期:2014-08-06 发布日期:2014-08-06
  • 通讯作者: 张世馥 E-mail:cklzhang@163.com

Expression of NonO protein during murine erythroleukemia cell differentiation induced by sodium butyrate

SANG Ting-ting  HU Jiang-jiang  XUE Jian-you  QI Wu-lin  ZHAO Fu-kun  ZHANG Shi-fu*   

  1. School of Life Science, Zhejiang SciTech University, Hangzhou 310018, China
  • Received:2013-12-13 Revised:2014-03-16 Online:2014-08-06 Published:2014-08-06
  • Contact: ZHANG Shi-fu E-mail:cklzhang@163.com

摘要:

目的 探讨NonO蛋白在丁酸钠诱导小鼠红白血病(MEL)细胞向红细胞系分化过程中的表达变化。方法 利用联苯胺染色观察丁酸钠诱导MEL细胞红细胞系分化情况,通过Western blotting、免疫细胞化学检测NonO蛋白在丁酸钠诱导MEL细胞分化的过程中表达量的变化,PCR技术检测NonO基因在RNA水平上的表达变化,免疫细胞化学技术检测NonO蛋白在MEL细胞中的定位。 结果 发现NonO在丁酸钠诱导MEL细胞向红细胞系分化过程中在基因水平和蛋白水平上均上调表达,NonO蛋白在MEL细胞中定位于细胞质和细胞核。结论 NonO蛋白与丁酸钠诱导MEL细胞分化密切相关。

关键词: NonO蛋白, 小鼠红白血病细胞, 丁酸钠, 红细胞系, 分化, 免疫组织化学, 免疫印迹法

Abstract:

Objective To study NonO protein expression changes in murine erythroleukemia (MEL) cell differentiation induced by sodium butyrate.Methods Benzidine staining was used to test sodium butyrate-induced erythroid differentiation of MEL cells. We detected NonO protein expression changes in MEL cell differentiation induced by sodium butyrate and NonO protein localization in MEL cells by Western blotting and immunocytochemistry. Furthermore, we applied PCR technique to detect NonO RNA expression in differentiation process. Results We found that NonO protein was upregulated at gene and protein levels in the erythroid differentiation process of MEL cells induced by sodium butyrate and located in cytoplasm and nucleus in MEL cells. Conclusion These results show that NonO protein is closely related with MEL cell differentiation induced by sodium butyrate,which may provide important clues for further study of the mechanism of leukemia.

Key words: NonO protein, Murine erythroleukemia cell, Sodium butyrate, Erythroid, Differentiation, Immunohistochemistry, Western blotting