解剖学报 ›› 2014, Vol. 45 ›› Issue (4): 452-456.doi: 10.3969/j.issn.0529-1356.2014.04.003

• 神经生物学 • 上一篇    下一篇

创伤后应激障碍大鼠海马神经元凋亡中Caspase-12的表达

刘虹 韩芳 石玉秀*   

  1. 中国医科大学基础医学院组织学与胚胎学教研室,沈阳 110001
  • 收稿日期:2013-09-04 修回日期:2013-12-03 出版日期:2014-08-06 发布日期:2014-08-06
  • 通讯作者: 石玉秀 E-mail:shiyuxiu@163.com
  • 基金资助:

    内质网分子伴侣Grp78、Grp94及CRT对PTSD海马神经元内质网径路细胞凋亡及钙稳态调控的分子伴侣

Expression of Caspase-12 in cellular apoptosis of hippocampus in the rat model of posttraumatic stress disorder

LIU Hong  HAN Fang  SHI Yu-xiu*   

  1. Department of Histology and Embryology, Basic Medical Sciences College, China Medical University, Shenyang 110001, China
  • Received:2013-09-04 Revised:2013-12-03 Online:2014-08-06 Published:2014-08-06

摘要:

 目的 观察创伤后应激障碍(PTSD)大鼠海马神经元凋亡和Caspase-12表达的变化。方法 采用单一延长应激(SPS)方法刺激大鼠建立PTSD模型,健康雄性Wistar大鼠随机分为SPS 1d、4d、7d组和正常对照组。应用透射电子显微镜观察PTSD大鼠海马神经元的形态学改变,采用免疫组织化学、免疫印迹法和RT-PCR技术检测海马神经元Caspase-12表达的变化。 结果 SPS后,海马神经元发生了凋亡的形态学改变,Caspase-12的表达于刺激后4d最多。结论 PTSD激活凋亡因子Caspase-12启动海马神经元经内质网途径细胞凋亡,可能是PTSD患者海马萎缩的一个病理生理学基础。

关键词: 创伤后应激障碍, 海马, 神经元, 免疫印迹法, 大鼠

Abstract:

Objective To observe changes of apoptosis and Caspase-12 expression in hippocampus of the rat model of posttraumatic stress disorder (PTSD).Methods Single prolonged stress (SPS) was used to duplicate a rat model of PTSD.Male Wistar rats were randomly divided into 1, 4, 7 days groups after exposure to SPS and a normal control group. Transmission electron microscopy was used to observe morphologic changes of the hippocampus neurons. The expression of Caspase-12 was detected by immunohistochemistry, Western blotting and RT-PCR. Results After SPS, the hippocampus neurons had characteristic morphologic changes of apoptosis, and the expression of Caspase-12 reached the peak at SPS 4d.Conclusion PTSD activates the ERS-mediated apoptosis in hippocampus via Caspase-12, which may be a pathophysiology base of the atrophy of the hippocampus in PTSD.

Key words: Posttraumatic stress disorder, Hippocampus, Neuron, Wstern blotting, Rat