解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (4): 475-479.doi: 10.3969/j.issn.0529-1356.2014.04.007

• 神经生物学 • 上一篇    下一篇

三叶因子3在大鼠腺垂体嗜色细胞中的定位

张文静1 孙琳2 王静3 张静1 吴靖芳1*   

  1. 1.河北北方学院组织学胚胎学教研室；2.解剖学教研室，河北 张家口 075000; 3.迁安市妇幼保健院妇科，河北 唐山 064400
  • 收稿日期:2013-05-23 修回日期:2013-11-04 出版日期:2014-08-06 发布日期:2014-08-06
  • 通讯作者: 吴靖芳 E-mail:wjfxg@163.com
  • 基金资助:

    三叶因子家族对胃溃疡形成及愈合的调控作用及机制

Location of trefoil factor 3 in chromophil cells of rat adenohypophysis

ZHANG Wen-jing1 SUN Lin2 WANG Jing3 ZHANG Jing1 WU Jing-fang 1*   

  1. 1.Department of Histology and Embryology；2.Department of Anatomy，Hebei North University，Hebei Zhangjiakou 075000，China; 3.Department of Gynecology in Maternal and Child Health Hospital of Qian’an City in Hebei Province，Hebei Tangshan 064400，China
  • Received:2013-05-23 Revised:2013-11-04 Online:2014-08-06 Published:2014-08-06
  • Contact: WU Jing-fang E-mail:wjfxg@163.com

摘要：

目的 探讨三叶因子3（TFF3）在腺垂体远侧部嗜酸性细胞和嗜碱性细胞中的表达，明确TFF3在远侧部的分布。 方法 采用相邻切片的免疫组织化学染色，在相邻切片上分别显示TFF3/生长激素（GH)、TFF3/催乳素（PRL)、TFF3/促甲状腺激素（TSH）、TFF3/促肾上腺皮质激素（ACTH）、TFF3/卵泡刺激素（FSH）、TFF3/黄体生成素（LH）的表达。 结果 TFF3和各嗜色细胞的免疫反应产物为棕黄色，主要位于细胞质，ACTH免疫阳性信号在胞膜也有表达，主要分布在腺垂体的远侧部。邻片显示TFF3存在于部分GH、PRL、TSH、ACTH、FSH、LH细胞，分别占19.4%、22.4%、9.2%、6.5%、35.7%、8.3%，以FSH最多，PRL、GH次之。 结论 垂体远侧部TFF3可分别表达于GH、PRL、TSH、ACTH、FSH、LH细胞。

关键词: 三叶因子3, 垂体远侧部, 嗜色细胞, 嗜酸性细胞, 嗜碱性细胞, 共存, 免疫组织化学, 大鼠

Abstract:

Objective To investigate the distribution and expression of trefoil factor 3(TFF3) in the eosinophilic cells and basophilic cells in the pars distalis of the rat pituitary. Methods The immunohistochemiscal staining technique was used to show the coexpression of TFF3/ growth hormone (GH)，TFF3/ prolactin (PRL)，TFF3/ thyrotroph (TSH)，TFF3/ adrenocorticotropin (ACTH)，TFF3/ follicle stimulating hormone (FSH)，TFF3/ luteinizing hormone (LH) in two contiguous slices. Results The immunoreactive products of TFF3 and chromophil cells were brown granules，mainly expressed in cytoplasm.ACTH positive signals were expressed in the cell membrane and mainly located in the pars distalis hypophyseos.TFF3 existed in parts of GH，PRL，TSH，ACTH，FSH and LH cells in contiguous slices, accounting for 19.4%, 22.4%, 9.2%, 6.5%, 35.7%, 8.3%, respectively, in which FSH was the most, PRL and GH were less. Conclusion TFF3 expresses in GH，PRL，TSH，ACTH，FSH, and LH cells in the pars distalis，which enriches the morphological data for the location of TFF3 in gland cells of pars distalis hypophyseos.

Key words: Trefoil factor 3, Pars distalis hypophyseos, Chromophil cell, Acidophil, Basophil, Coexistence, Immunohistochemistry, Rat