›› 2007, Vol. 38 ›› Issue (1): 38-42.doi:
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彭会明;刘仁刚*;罗贤雯;周洁萍
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关键词: CD81, 星形胶质细胞, 增殖, 细胞周期, 四甲基噻唑蓝比色法, 大鼠
Abstract: Objective To investigate the effect of antiCD81(antibodys against CD81) on the proliferation of astrocytes. Methods Purified astrocytes from newborn rats’ cerebral cortex were divided into 6 groups and added with antiCD81 different concentrations(0,0.1,0.5,1,5,10mg/L). The activity of astrocytes was tested by methyl thiazolyl terazolium(MTT). Three significative groups were chosen based on MTT result and added with antiCD81 of different concentrations(0,0.5,5mg/L). After administration for 24 hours, the cell cycle of the astrocytes was measured by flow cytometer. The corresponding data were analyzed with SPSS statistical software. Results 1By MTT, the average optical density(AOD) values of astrocytes were reduced after administration with antiCD81 of different concentrations for 24 hours, that is, the number of astrocytes was reduced, which indicated antiCD81 inhibited the proliferation of astrocytes and the effect showed a dosedependent pattern.2By cell cycle analysis, a progressive dosedependent decrease was found in the index of cells in G0/G1 phase and an increase in S phase. Such as, the index of cells in G0/G1 phase, was 82.73 in 0, is 8216 in 0.5mg/L, was 78.58 in 5mg/L. Conclusion AntiCD81 inhibits the proliferation of astrocytes and the number of astrocytes is reduced.
Key words: CD81, Astrocyte, Proliferation, Cell cycle, MTT, Rat
中图分类号:
R322.811
彭会明;刘仁刚;罗贤雯;周洁萍. 抗CD81抗体对星形胶质细胞增殖的抑制作用[J]. , 2007, 38(1): 38-42.
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