解剖学报

解剖学报 ›› 2015, Vol. 46 ›› Issue (6): 819-823.doi: 10.16098/j.issn.0529-1356.2015.06.016

• 组织学胚胎学发育生物学 • 上一篇    下一篇

卵泡刺激素与卵泡刺激素及黄体生成素共同干预对玻璃化冻存小鼠卵巢组织血管内皮生长因子表达的影响

陈杰1,常青2,杨延周3,裴秀英3,黑长春3,于佳3,孙苗3,王燕蓉3   

  1. 1. 内蒙古医科大学人体解剖学教研室，呼和浩特 010000; 2. 宁夏医科大学生育力保持教育部重点实验室，宁夏生殖与遗传重点实验室，银川 750004; 3. 宁夏医科大学人体解剖学与组织学胚胎学系，银川 750004
  • 收稿日期:2015-04-23 修回日期:2015-06-17 出版日期:2015-12-06 发布日期:2015-12-06
  • 通讯作者: 王燕蓉 E-mail:4083304@163.com
  • 基金资助:

    促性腺激素干预在卵巢玻璃化冻存中的抗凋亡及促移植后血管重建的作用

Effect of intervention of follicle-stimulating hormone/ follicle-stimulating hormone and luteinizing hormone on the expression of vascular endothelial growth factor in the vitrification mouse ovary

CHEN Jie1 CHANG Qing 2,3 YANG Yan-zhou 2,3 PEI Xiu-ying2 HEI Chang-chun3 YU Jia2 SUN Miao2 WANG Yan-rong 2,3*   

  1. 1. Department of Human Anatomy,Inner Mongolia Medical University,Hohhot 010000，China; 2.Key Laboratory of Fertility Maintenance, Ministry of Education, Ningxia Medical University & Key Laboratory of Reproduction and Genetics, Ningxia,Yinchuan 750004，China;  3.Department of Human Anatomy and Histoembrylolgy, Ningxia Medical University,Yinchuan 750004，China

  • Received:2015-04-23 Revised:2015-06-17 Online:2015-12-06 Published:2015-12-06
  • Contact: WANG Yan-rong E-mail:4083304@163.com

摘要：

目的 通过在玻璃化冻存全程给予小鼠卵巢组织卵泡刺激素（FSH）及FSH和黄体生成素（LH）共同干预，观察冻融卵巢组织的形态学改变以及两种激素干预对冻存卵巢组织血管内皮生长因子（VEGF）表达的影响，寻找最佳的提高冻融卵巢组织卵泡存活及VEGF表达的激素干预方式。 方法 4周龄C57BL/6J小鼠卵巢组织分为新鲜对照组（CG），玻璃化冻存对照组（VCG），300IU/L FSH全程干预玻璃化冻存组（OG-FSH），以及150IU/L FSH+150IU/L LH全程干预玻璃化冻存组(OG-FSH+LH)，每组30个卵巢样本。通过常规组织学、Western blotting技术，观察并分析各组卵巢组织形态结构改变及VEGF蛋白表达量；通过荧光定量PCR技术(Real-time PCR)检测VEGF mRNA表达情况。 结果 OG-FSH+LH 组正常卵泡百分比最高，且显著高于OG-FSH组（P<0.05）；VEGF蛋白表达量在OG-FSH+LH组显著高于OG-FSH组（P<0.05）；荧光定量PCR检测结果表现为VEGF mRNA表达量在OG-FSH+LH组最高，其次为OG-FSH组，最低是VCG组（P<0.05）。 结论 玻璃化冻存全程添加FSH+LH的干预方式较单独FSH干预具有更高正常卵泡百分比和更佳的VEGF蛋白表达。

关键词: 卵泡刺激素, 黄体生成素, 血管内皮生长因子, 免疫印迹法, 实时定量聚合酶链反应, 小鼠

Abstract:

Objective To investigate the best way of hormone intervention for protecting the vitrification ovarian tissues via observation of the morphological changes of ovary and the expression of vascular endothelial growth factor (VEGF）after the intervention by follicle-stimulating hormone（FSH）/FSH+ luteinizing hormone (LH）in the whole process of vitrification. Methods Four-week-old C57BL/6J mice were divided into four groups:the fresh control group(CG),the vitrification control group(VCG), the vitrification group intervention by 300 IU/L FSH(OG-FSH) and the vitrification group intervention by 150 IU/L FSH+150 IU/L LH(OG-FSH+LH),30 ovary samples per group. The changes of morphological structure of ovary,the expression of VEGF protein and mRNA in every group were observed and analyzed using routine histology, Western blotting and Real-time PCR. Results The percentage of normal follicles was the highest in OG-FSH+LH group,and significantly higher in OG-FSH+LH group than in OG-FSH group (P<0.05). The expression of VEGF protein was higher in OG-FSH+LH group than OG-FSH group(P<0.05).The order of intensities of expression for VEGF mRNA was OG-FSH+LH group> in OG-FSH group>in VCG group (P<0.05). Conclusion The best intervention way is FSH+LH interpose in the whole process of vitrification, which is in favour of the expression of VEGF.

Key words: Follicle-stimulating hormone, Luteinizing hormone, Vascular endothelial growth factor, Western blotting, Real-time PCR, Mouse