何首乌饮对衰老大鼠生精细胞线粒体凋亡通路关键基因表达的影响

doi:10.16098/j.issn.0529-1356.2017.03.007

解剖学报 ›› 2017, Vol. 48 ›› Issue (3): 287-295.doi: 10.16098/j.issn.0529-1356.2017.03.007

何首乌饮对衰老大鼠生精细胞线粒体凋亡通路关键基因表达的影响

安福丽¹王玉娟¹ 刘昊坤² 杜云帆²秦瑄希²牛嗣云^3* 高福禄^4*

1. 河北省中医院药学部，石家庄 050011; 2. 中国人民解放军军事交通学院九连，天津 300000; 3. 河北大学医学院组织学胚胎学教研室，河北保定 071000; 4. 河北师范大学生命科学学院，石家庄 050024

收稿日期:2016-11-24 修回日期:2017-01-13 出版日期:2017-06-06 发布日期:2017-09-19
通讯作者: 牛嗣云;高福禄 E-mail:nsy1688@163.com
基金资助:
基于表观遗传学理论研究补肾中药对肾精亏虚雄性大鼠生殖衰老调控的DNA甲基化机制

Effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats’spermatogenic cells#br#

AN Fu-li¹WANG Yu-juan¹ LIU Hao-kun²DU Yun-fan² QIN Xuan-xi² NIU Si-yun ^3* GAO Fu-lu^4*

1. Department of Pharmacy,Chinese Traditional Medicine Hospital of Hebei Province, Shijiazhuang 050011, China; 2. Military Transportation University of PLA, Tianjin 300000, China; 3. Department of Embryology Organization, Hebei University, Hebei Baoding 071000, China; 4. School of Life Science, Hebei Normal University, Shijiazhuang 050024, China

Received:2016-11-24 Revised:2017-01-13 Online:2017-06-06 Published:2017-09-19
Contact: NIU Si-yun;GAO Fu-lu E-mail:nsy1688@163.com

摘要/Abstract

摘要：

目的探讨何首乌饮对衰老大鼠睾丸生精细胞线粒体凋亡通路关键基因表达的影响。方法选用12月龄Wistar雄鼠45只，随机分为3组：青年对照组、自然衰老组、何首乌饮组，每组15只，何首乌饮组和自然衰老组于16个月开始分别灌胃何首乌饮和等量蒸馏水，连续60d。青年对照组于12月龄，其余两组于18月龄分别进行实验，通过Real-time PCR、免疫荧光染色和免疫印迹法，观察各组大鼠睾丸组织线粒体凋亡通路关键基因DR6、BAX、Caspase-3、Cyt-C、14-3-3σ的表达变化。结果自然衰老大鼠线粒体凋亡通路关键基因14-3-3σ 表达明显低于青年对照组，DR6、BAX、Caspase-3、Cyt-C表达明显高于青年对照组；而何首乌饮用药后能明显逆转上述现象。结论何首乌饮提高衰老大鼠精子质量与线粒体凋亡通路有关。

关键词: 何首乌饮, 睾丸, 线粒体凋亡通路, 实时定量聚合酶链反应, 免疫荧光, 免疫印迹法, 衰老, 大鼠

Abstract:

Objective To investigate the effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats’ spermatogenic cells. Methods Forty-five 12-month-old male Wistar rats were randomly divided into three groups: young control group(YCG), natural aging group(NAG) and Heshouwuyin group(SWYG), 15 rats per group. From 16monthold, SWYG and NAG were intragastrically given Heshouwuyin and equal amount of normal saline respectively. Gavage treatment kept for 60 days. YCG at 12-month-old and the other two groups at 18-month-old entered experiment respectively. Real-time PCR, immunofluorescence staining, and Western blotting were used to detect the expression of DR6, BAX, Caspase-3, Cyt-C and 14-3-3σ. Results The expression of 14-3-3σ, a key factor in the mitochondrial apoptotic pathway, was significantly lower and the expressions of DR6, BAX, Caspase-3, and Cytc were significantly higher in the NAG than in the YCG, but intervention with Heshouwuyin significantly reversed this phenomenon. Conclusion Heshouwuyin improves the spermatogenic function of the testes by regulating expression of key genes in the mitochondrial apoptosis pathway.

Key words: Heshouwuyin, Testis, Mitochondrial apoptosis pathway, Real-time PCR, Immunofluorescence, Western blotting, Aging, Rat

安福丽王玉娟刘昊坤杜云帆秦瑄希牛嗣云高福禄. 何首乌饮对衰老大鼠生精细胞线粒体凋亡通路关键基因表达的影响[J]. 解剖学报, 2017, 48(3): 287-295.

AN Fu-li WANG Yu-juan LIU Hao-kun DU Yun-fan QIN Xuan-xi NIU Si-yun GAO Fu-lu. Effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats’spermatogenic cells#br#[J]. Acta Anatomica Sinica, 2017, 48(3): 287-295.

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何首乌饮对衰老大鼠生精细胞线粒体凋亡通路关键基因表达的影响

Effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats’spermatogenic cells#br#

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