凋亡抑制因子5在卵巢癌中的表达及临床意义

doi:10.16098/j.issn.0529-1356.2017.03.009

解剖学报 ›› 2017, Vol. 48 ›› Issue (3): 303-309.doi: 10.16098/j.issn.0529-1356.2017.03.009

凋亡抑制因子5在卵巢癌中的表达及临床意义

王娟¹ 花晓棠^2*

1. 南通市如东县人民医院妇产科，江苏南通 226400； 2. 南通大学附属医院分院妇科，江苏南通 226001

收稿日期:2016-10-12 修回日期:2017-02-09 出版日期:2017-06-06 发布日期:2017-09-19
通讯作者: 花晓棠 E-mail:ntfyhxt@163.com

Expression and significance of apoptosis inhibitor 5 in ovarian cancer

WANG Juan¹ HUA Xiao-tang ^2*

1. Department of Gynecology and Obstetrics, Nantong Rudong County People’s Hospital, Jiangsu Nantong 226400, China;
2. Department of Gynecology, Affiliated Branch Hospital of Nantong University,Jiangsu Nantong 226001, China

Received:2016-10-12 Revised:2017-02-09 Online:2017-06-06 Published:2017-09-19
Contact: HUA Xiao-tang E-mail:ntfyhxt@163.com

摘要/Abstract

摘要：

目的检测凋亡抑制因子5（API5）在卵巢癌中的表达，分析其与临床病理特征的关系。方法 Western blotting方法检测2例正常卵巢组织及6例不同级别的卵巢癌组织的表达，组织化学方法检测10 例正常卵巢、119 例卵巢癌(浆液性腺癌61例、黏液性癌5例、内膜样癌10例、透明细胞癌10例及未分化癌33例) 组织中API5的表达，分析其表达与临床病理参数及5年生存率之间的相关性，细胞转染及凋亡流式细胞分析检测API5及顺铂对卵巢癌细胞的作用。结果 API5在卵巢癌组织中的表达高于正常卵巢组织，API5的表达与临床病例特征之间密切相关，Kaplan-Meier生存曲线揭示API5表达高的卵巢癌患者较低表达患者生存率低，流式细胞术分析显示，抑制API5的表达能增加顺铂对卵巢癌细胞的敏感性。结论 API5的异常表达与卵巢癌的发生密切相关，提示API5在预后评价中具有潜在的临床应用价值，有可能成为卵巢癌治疗的潜在靶点。

关键词: 卵巢癌, 凋亡抑制因子5, 顺铂, 免疫组织化学, 人

Abstract:

Objective The study is designed to investigate the expression of apoptosis inhibitor 5（API5）in ovarian cancer（OC）, and to analysis its association with clinical pathologic variables. Methods API5 expression was evaluated by Western blotting in two normal ovarian tissues and six different fresh OC tissues. Immunohistochemistry analysis was performed on formalin-fixed paraffinembedded sections of 10 normal tissues and 119 cases of OCs (including 61 serous papillary adenocarcinoma, 10 endometrioid adenocarcinoma, 10 clear cell carcinoma, 5 mucinous papillary carcinoma, and 33 specimens were classified as undifferentiated carcinoma). SiRNA transfection and flow cytometry showed the impact of API5 and cisplatin on ovarian cancer cells. Results The expression of API5 was higher in the cancer samples compared with that in the normal ovary tissues. API5 was significantly associated with clinical pathologic variables(P<0.05). Kaplan-Meier curve showed that high expression of API5 was related to poor prognosis of OC patients than that low expression. Decreasing the expression of API5 enhanced EOC cell line’s sensibility to cisplatin by flow cytometry. Conclusion Our findings suggest that API5 is involved in the progression of OC and has a potential clinical application value in the assessment of prognosis, which may be a target of therapy in OC.

Key words: Ovarian cancer, Apoptosis inhibitor 5, Cisplatin, Immunohistochemistry, Human

王娟花晓棠. 凋亡抑制因子5在卵巢癌中的表达及临床意义[J]. 解剖学报, 2017, 48(3): 303-309.

WANG Juan HUA Xiao-tang. Expression and significance of apoptosis inhibitor 5 in ovarian cancer[J]. Acta Anatomica Sinica, 2017, 48(3): 303-309.

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凋亡抑制因子5在卵巢癌中的表达及临床意义

Expression and significance of apoptosis inhibitor 5 in ovarian cancer

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