百合水溶液对麻黄素致损伤小鼠肺组织的影响

doi:10.16098/j.issn.0529-1356.2017.06.019

摘要/Abstract

摘要：

目的探讨百合水溶液对麻黄素致损伤肺组织的影响。方法选出生10 d的小鼠72只，随机分为空白对照组、实验对照组和百合组3组。实验对照组和百合组采用递增剂量连续（1-5，5-10，11-15d）腹腔注射（2.0、4.0、6.0 g/L）麻黄素溶液15 d（每次0.2 mL，每天两次），百合组注射麻黄素1 h后，再灌胃0.2 mL百合水溶液，实验对照组灌胃等量的生理盐水，空白对照组腹腔注射和灌胃等量的生理盐水。用比色法检测肺组织SOD、CAT和GSH-Px活性及血浆MPO活性的变化，用石蜡包埋法和HE染色法观察小鼠肺组织结构的病理变化，用免疫组织化学法检测小鼠肺组织Bax、NF-κB蛋白表达的变化。结果实验对照组肺组织SOD、CAT和GSH-Px活性显著降低(P<0.01或P<0.05)，血浆MPO活性显著升高(P<0.01或P<0.05)，肺组织出现明显病理性损伤，肺泡上皮细胞局部降解、脱落，肺泡直径和肺泡隔厚度增大 (P<0.01)，肺组织Bax、NF-κB蛋白的阳性表达明显增强 (P<0.01或P<0.05)。与实验对照组相比较，百合组肺组织SOD、CAT和GSH-Px活性又升高 (P<0.01或P<0.05)，血浆MPO活性又显著降低（P<0.05)，肺组织损伤明显改善，肺泡上皮脱落减少，肺泡直径和肺泡隔厚度均又减小(P<0.01或P<0.05)，肺组织Bax、NF-κB蛋白的阳性表达明显减弱 (P<0.01或P<0.05)。结论百合水溶液能增强机体抗氧化能力，提高机体免疫力，抑制肺组织Bax、NF-κB蛋白的阳性表达和肺组织细胞凋亡，对麻黄素致损伤肺组织有一定的保护作用。

关键词: Lilium soup, Ephedrine, Lung, Histological structure, Antioxidant enzyme, Immunohistochemistry, Mouse

Abstract:

Objective To explore the effects of lilium soup on ephedrine-injured mice lung. Methods Totally 72 Kunming mice ten days after birth were used and randomly divided into 3 groups：nature control group，experimental control group and lilium group．Experimental control group and lilium group were intraperitoneally injected escalation doses (2.0 g/L，4.0 g/L，6.0 g/L) ephedrine 0.2 ml twice every day．And nature control group was injected the same amount of saline．One hour after injection of ephedrine, lilium group was intragastric administration with 0.2 ml lilium soup．Experimental control group and nature control group were intragastric administed with the same amount of saline．The change of superoxide dismutase (SOD)，hydrogen peroxidase (CAT) and glutathione peroxidase (GSH-Px) activity of lung and the activity of plasma myeloperoxidase (MPO) were determined by colorimetry．HE staining was used to observe the change of histological structure of lung．Immunohistochemistry was used to detect the change of Bax and nuclear factor-kappa B (NF-κB) expression in lung． Results The activities of SOD，CAT and GSH-Px of experimental control group were significantly lower than that of the nature control group (P<0.01 or P<0.05)．The activity of plasma MPO of experimental control group were significantly increaser than that of the nature control group (P<0.01 or P<0.05)．Lung histopathologic damage of experimental control group was obvious．The alveolar structure were disorder and epithelial cells were fall off．The alveolar diameter and thickness of the alveolar septum increased significantly than the nature control group (P<0.01)．The stained color and average optical density of Bax and NF-κB increased significantly in experimental control group than that of the nature control group (P<0.01 or P<0.05)．Compared with experimental control group，the activities of SOD，CAT and GSH-Px of lilium group increased (P<0.01 or P<0.05). The activity of plasma MPO of lilium group reduced，the alveolar diameter and thickness of the alveolar septum were reduced in lilium group (P<0.01 or P<0.05) wih lighter histopathologic damage of lung，the stained color and the average absorbance of lilium group were reduced(P<0.01 or P<0.05). Conclusion Lilium soup could have an protective effect on the damage of mice lung induced by ephedrine by increase in activities of antioxidant enzyme and improving the body immunity，regulating the metabolism，restraining Bax and NF-κB expression of lung tissue and cell apoptosis．

李重阳彭静俞诗源. 百合水溶液对麻黄素致损伤小鼠肺组织的影响[J]. 解剖学报, 2017, 48(6): 746-752.

LI Chong-yang PENG Jing YU Shi-yuan. Effects of lilium soup on lung histological structure in mice injured by ephedrine[J]. Acta Anatomica Sinica, 2017, 48(6): 746-752.

参考文献

［1］Li ChY，Gao XJ，Yu ShY，et al．Effects of heroin and ephedrine on the histological structure and ChAT activity of hypothalamus and hippocampus of filial mice［J］．Acta Anatomica Sinica，2009，40(6)：862-868．（in Chinese）

李重阳，高先军，俞诗源，等．注射海洛因、麻黄素对仔鼠下丘脑、海马组织结构及胆碱乙酰基转移酶活性的影响［J］．解剖学报，2009，40(6)：862-868．

［2］Tao YF，Zhao ZhH，Zhou H，et al. A comparative study of trazodone and buprenornhine in the treatment of protracted withdrawal syndrome in heroin addicts［J］. Chinese Journal of Drug Abuse Prevention and Treatment，2010，16(5)：256-257．(in Chinese)

陶用富，赵智慧，周豪，等. 曲唑酮与丁丙诺啡治疗海洛因依赖者稽延性戒断症状的对照研究［J］. 中国药物滥用防治杂志，2010，16(5)：256-257.

［3］Liu TT，Shi J，David HE，et al. A Meta-analysis of chinese herbal medicne in treatment of managed withdrawal from heroin［J］．Chinese Journal Drug Dependence，2011，20（4）：301-307．(in Chinese)

刘婷婷，时杰， David HE，等. 中药治疗海洛因依赖急性戒断症状的Meta 分析［J］. 中国药物依赖性杂志，2011，20（4）：301-307.

［4］Chen MZh，Chen ShY，Chen ChH. Inhibition of small complex prescription of salviamiltiorrhiza and radix astragali on relapse behavior in morphine dependent mice［J］．Chinese Journal of Drug Dependence，2008，17(1)：32-35．(in Chinese)

陈明珠，陈少雅，陈崇宏．丹参、黄芪小复方对吗啡依赖小鼠复吸行为的抑制作用［J］．中国药物依赖性杂志，2008，17(1)：32-35．

［5］Wei PT，Li ChY，Yu ShY，et al．The effect of Astragalus polysaccharide on histological structure and enzyme activity and Caspase-3 protein expression in the kidney of mice injured by ephedrine［J］．Acta Anatomica Sinica，2015，46(4)：564-571．（in Chinese）

魏娉婷，李重阳，俞诗源，等．黄芪(粗)多糖对麻黄素损伤小鼠肾组织结构和抗氧化物酶活性及Caspase-3蛋白表达的影响［J］．解剖学报，2015，46(4)：564-571．

［6］Hu HP，Zhang J，Gan YH，et al．Single taste fresh lily cough sedative and hypnotic effects of pharmacological experiment ［J］．LiShiZhen Medicine and Materia Medica Research，2006，17(9)：1704-1705．（in Chinese）

胡焕萍，张剑，甘银凰，等．单味新鲜百合止咳镇静催眠等作用药理实验［J］．时珍国医国药，2006，17(9)：1704-1705．

［7］Pei ShY，Zhao J，Guo Zh. Research progress on pharmacological action of polysaccharides from lily ［J］．China Pharmacy，2013，24(35)：3345-3347．（in Chinese）

裴淑艳，赵晋，郭忠．百合多糖的药理作用研究进展［J］．中国药房，2013，24(35)：3345-3347．

［8］Feng HL，Li ChY，Yu ShY，et al．Effects of heroin on SOD and CAT activity and MDA content and histological structure in the lungs of the filial mice and mother mice［J］．Acta Anatomica Sinica，2009，40(2)：288-294．（in Chinese）

冯红丽，李重阳，俞诗源，等．海洛因对仔鼠和母鼠肺中SOD、CAT活力和MDA含量及组织结构的影响［J］．解剖学报，2009，40(2)：288-294．

［9］Meng R，Li ChY，Yu ShY，et al．Protection of angelica sinensis polysaccharide on liver histological structure in mice injured by ephedrine［J］．Acta Anatomica Sinica，2015，46(3)：379-386．(in Chinese

孟茹，李重阳，俞诗源，等．当归（粗）多糖对麻黄素肝组织损伤的保护作用［J］．解剖学报，2015，46(3)：379-386．

［10］Wang YM．Free radical and glutathione peroxidase ［J］．Pharmaceutical Journal of Chinese People’s Liberation Army，2005，21(5)：369-371．（in Chinese）

王咏梅．自由基与谷胱甘肽过氧化物酶［J］．解放军药学学报，2005，21(5)：369-371．

［11］Dai T，Yi ZhF，Wang LY．Progress in clinical application of reduced glutathione ［J］．Journal of Chengde Medical College，2014(5)：432-435．（in Chinese）

代涛，尹志峰，王良友．还原型谷胱甘肽临床应用研究进展［J］．承德医学院学报，2014(5)：432-435．

［12］He FCh，Ying HQ，An YH．The serum and liver SOD，MDA levels in morphine addicted mice［J］．Journal of Zhengzhou University(Medical Sciences)，2005，40(4)：694-695．（in Chinese）

贺付成，尹惠卿，安玉会．吗啡成瘾小鼠血清及肝脏SOD和MDA含量的变化［J］．郑州大学学报（医学版），2005，40(4)：694-695．

［13］Peng J，Li ChY，Liu TT，et al. Effects of ephedrine on histological structure and its mechanism in mice lung［J］．Acta Anatomica Sinica，2016，47(4)：521-527．(in Chinese) 彭静，李重阳，刘婷婷，等．麻黄素对小鼠肺组织结构的影响及其机理研究［J］．解剖学报，2016，47(4)：521-527.

［14］He ChL，Chen LSh, Ren FL．Study of effect of Lilium extract on scavenging hydroxyl free radical［J］．Physical Testing and Chemical Analysis Part B Chemical Analysis，2005，41(8)：558-560．（in Chinese）

何纯莲，陈腊生，任凤莲．药用百合提取液对羟自由基清除作用的研究［J］．理化检验：化学分册，2005，41(8)：558-560．

［15］Junichi K，Atsunori N，Ryujiro S，et al．Carbon monoxide-saturated preservation solution protects lung grafts from ischemia-reperfusion injury［J］．J Thorac Cardiovasc Surg，2008，136(4)：1067-1075．

［16］Zhang JL，Qin WT，Lü WH，et al. Inhibitory effect of kukoamine responses in mice with sepsls［J］. Chinese Critical Care Medicine，2014，26(7)：493-497．(in Chinese)

张锦丽，秦魏婷，吕汪洄，等．苦柯胺B对脓毒症小鼠肺脏炎症反应的抑制作用［J］.中华危重病急救医学，2014，26(7)：493-497．

［17］Wu ChY，Zhang Zh，Gu WW，et al. Effect of puerarin on activity of MPO and expression of ICAM-1 in ratr with acute lung injury［J］.China Pharmacist，2013，16(11)：1653-1655．

吴春艳，张智，谷玮玮，等．葛根素对急性肺损伤大鼠肺组织MPO活性及ICAM-1表达的影响［J］．中国药师，2013，16(11)：1653-1655．

［18］Wei H．The study about the effects and mechanism on lung development of marginal vitamin A deficiency beginning from embronic period in young rats［D］．Chongqing：Chongqing Medical University，2008．(in Chinese)

魏华．孕期边缘型维生素A缺乏对仔鼠肺发育的影响及机制研究［D］．重庆：重庆医科大学，2008．

［19］Wang X．A study on the toxicity and drug protection of amphetamine-type stimulants［D］．Chengdu：Sichuan University，2003．（in Chinese）

王雪．苯丙胺类兴奋剂的毒性及药物保护的研究［D］．成都：四川大学，2003．

［20］Wang XJ，Wei DB，Wei L，et al．Characteristics of pulmonary acinus structure in the plateau zokor myospalax baileyi and plateau pika ochotona curniae［J］．Acta Zoologica Sinica，2008，54(3)：531-539．(in Chinese)

王晓君，魏登邦，魏莲，等．高原鼢鼠和高原鼠兔肺细叶的结构特征［J］．动物学报，2008，54(3)：531-539．

［21］Yang TH，Jia M，Mei QB．Immunoloregulation effect of polysaccharide isolated from Angelica Sinensis on mouse［J］．Chinese Traditional Patent Medicine，2005，27(5):563-565．(in Chinese)

杨铁虹，贾敏，梅其炳．当归多糖对小鼠免疫功能的调节作用［J］．中成药，2005，27(5):563-565．

［22］Karch J，Kwong JQ，Burr AR，et al．Bax and Bak function as the outer membrane component of the mitochondrial permeability pore in regulating necrotic cell death in mice［J］．Elife，2013，2：e00772.

［23］Li WL，Liang X，Zhang W，et al. Expression of apoptosis-related genes in mouse lungs induced by phosgene ［J］．Carcinogenesis,Teratogenesis & Mutagenesis，2010,22(6)：436-439．（in Chinese）

李文丽，梁欣，张伟，等．光气致小鼠肺组织细胞凋亡相关基因的表达［J］．癌变·畸变·突变，2010,22(6)：436-439．

［24］Hu J，Yu M，Tang Y， et al．Effects of glucocorticoid on RAGE-NF-κB pathway in hyperoxia-induced lung tissues of neonatal rats［J］．Chinese Journal of Contemporary Pediatrics，2015,17(1)：81-85．（in Chinese）

胡剑，俞敏，唐云，等．糖皮质激素对高氧诱导新生大鼠肺组织RAGE-NF-κB通路的影响［J］．中国当代儿科杂志，2015,17(1)：81-85．