解剖学报 ›› 2017, Vol. 48 ›› Issue (5): 590-594.

• 组织学胚胎学发育生物学 • 上一篇    下一篇

幼年特发性关节炎患儿关节滑膜组织中趋化因子CXCL10及其受体CXCR3的表达及意义

宫相翠1* 管慧2 刘杰1   

  1. 1. 青岛市妇女儿童医院内科门诊; 2. 青岛市妇女儿童医院血液科, 青岛 266034
  • 收稿日期:2017-03-03 修回日期:2017-04-06 出版日期:2017-10-06 发布日期:2017-10-06
  • 通讯作者: 宫相翠 E-mail:gongxiangcui8@163.com

Significance of CXCL10/ CXCR3 expression in the synovium of children with juvenile idiopathic arthritis

GONG Xiang-cui1* GUAN Hui2 LIU Jie1   

  1. 1. Department of Internal Medicine; 2. Blood Specialty,Qingdao Women and Children’s Hospital, Qingdao 266034,China
  • Received:2017-03-03 Revised:2017-04-06 Online:2017-10-06 Published:2017-10-06
  • Contact: GONG Xiang-cui E-mail:gongxiangcui8@163.com

摘要:

目的 检测趋化因子CXCL10 及其受体CXCR3 在幼年特发性关节炎( JIA) 患儿关节滑膜组织中的表达,探讨其在JIA 发病机制中的作用。方法 通过免疫组织化学方法,检测12 例JIA 患儿和4 例非JIA 患儿关节滑膜组织中CXCL10 和CXCR3 的表达; 通过半定量RT-PCR 方法,检测CXCR3 在JIA 患儿和对照幼儿关节滑膜组织中mRNA 水平的表达。结果 免疫组织化学结果表明,CXCL10 / CXCR3 在JIA 患儿及对照组阳性表达差异有显著性( P < 0. 05) ; CXCR3 在JIA 患儿关节滑膜组织中mRNA 表达水平( CXCR3∶ GAPDH 为2. 26 ± 1. 55) 显著高于对照组( 0. 66 ± 0. 44) ,两者差异有显著性( P < 0. 05) 。结论 趋化因子CXCL10 及其受体CXCR3 在幼年特发性关节炎( JIA) 的发病中可能起到了重要的作用。

关键词: CXCL10, CXCR3, 幼年特发性关节炎, 免疫组织化学, 儿童

Abstract:

Objective To investigate the expression of chemokine CXCL10 and its receptor CXCR3 in synovium of children with juvenile idiopathic arthritis ( JIA) and to explore the role of CXCL10 and CXCR3 in the pathogenesis of JIA. Methods Immunohistochemical method was used to detect CXCL10 / CXCR3 expression in the synovium of 12 cases of children with JIA and 4 cases of non JIA children. Semi quantitative RT-PCR was used to detect CXCR3 mRNA expression in children with JIA and control. Results There were significant differences in CXCL10 / CXCR3 expression between children with JIA and control group ( P < 0. 05) . CXCR3 mRNA expression in children with JIA ( CXCR3 ∶ GAPDH 2. 26 ± 1. 55) was significantly higher than that in the control group ( 0. 66 ± 0. 44,P < 0. 05) . Conclusion CXCL10 and CXCR3 may play important roles in the pathogenesis of juvenile idiopathic arthritis ( JIA) .

Key words: CXCL10, CXCR3, Juvenile idiopathic arthritis, Immunohistochemistry, Child

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