解剖学报

解剖学报 ›› 2017, Vol. 48 ›› Issue (5): 595-599.doi: 10.16098/j.issn.0529-1356.2017.05.017

• 组织学胚胎学发育生物学 • 上一篇    下一篇

维生素D3对自然流产模型小鼠胚胎丢失率及外周血免疫细胞的影响

徐慧1* 王玉兰2 宋晓晖1 黄娟3   

  1. 1. 华中科技大学同济医学院附属武汉儿童医院（武汉市妇幼保健院）妇产科，武汉 430014; 2.武汉市中心医院妇产科，武汉 430014; 3. 华中科技大学同济医学院附属武汉儿童医院（武汉市妇幼保健院）病理科，武汉 430014
  • 收稿日期:2016-12-29 修回日期:2017-01-17 出版日期:2017-10-06 发布日期:2017-10-06
  • 通讯作者: 徐慧 E-mail:xuhui3583@163.com

Influence of vitamin D3 on embryo loss rate and peripheral immune cells in mouse model of spontaneous abortion

XU Hui1* WANG Yu-lan2 SONG Xiao-hui1 HUANG Juan3   

  1. 1. Department of Gynaecology and Obstetrics, Wuhan Children’s Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Scieme and Technology,  Wuhan 430014, China; 2. Department of Gynaecology and Obstetrics, Central Hospital of Wuhan, Wuhan 430014, China; 3. Department of Pathology, Wuhan Children’s Hospital (Wuhan Maternal and Child Healthcare Hospital),  Tongji Medical College, Huazhong University of Scieme and Technology, Wuhan 430014, China
  • Received:2016-12-29 Revised:2017-01-17 Online:2017-10-06 Published:2017-10-06
  • Contact: XU Hui E-mail:xuhui3583@163.com

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摘要：

目的 探讨维生素D3（VitD3）对自然流产模型小鼠胚胎丢失率及外周血免疫细胞的影响。 方法 采用CBA/J×BALB/c建立正常妊娠小鼠模型，采用CBA/J×DBA/2建立自然流产小鼠模型，实验动物分5组：正常组、自然流产组、VitD3低剂量组、中剂量组和高剂量组，每组15只。VitD3各组自妊娠第1天开始腹腔注射VitD3溶液，单次VitD3给药剂量分别为1μg、4μg、16μg。记录和观察各组小鼠胚胎丢失情况，检测各组血清调节性T细胞（Treg）细胞因子：白细胞介素-10（IL-10），辅助性T细胞17（Th17）细胞因子：白细胞介素-17A（IL-17A）以及各组外周血Treg、Th17。 结果 与正常组相比，自然流产组胚胎丢失率显著升高（χ2=16.045，P＜0.05）；与自然流产组比较，VitD3低剂量组、中剂量组和高剂量组胚胎丢失率显著降低（χ2=5.881、13.704、15.663，P＜0.05）。与正常组相比，自然流产组Treg、IL-10、Treg/Th17、IL-10/IL-17A显著降低，Th17、IL-17A显著升高，差异均有统计学意义（P＜0.05）；与自然流产组比较，VitD3低剂量组、中剂量组和高剂量组Treg、IL-10、Treg/Th17、IL-10/IL-17A显著升高，Th17、IL-17A显著降低，差异均有统计学意义（P＜0.05）。 结论 VitD3能够降低自然流产模型小鼠胚胎丢失率，可能与调节Treg/Th17平衡有关。

关键词: 维生素D3, 自然流产, 胚胎丢失, 调节性T细胞/辅助性T细胞17, 流式细胞术, 小鼠

Abstract:

Objective To explore the influence of vitamin D3 (VitD3) on embryo loss rate and peripheral immune cells in a mouse model of spontaneous abortion. Methods CBA/J×BALB/c was adopted to establish the mouse model of normal pregnancy, while CBA/J×DBA/2 was adopted to establish the mouse model of spontaneous abortion. There were totally 5 groups: normal group, spontaneous abortion group, VitD3 low dose group, middle dose group and high dose groups. The VitD3 groups were treated by VitD3 solution diluted in saline with intraperitoneal injection of 1μg, 4μg, 16μg, respectively, 15 cases per group. The embryo loss situation in each group was recorded and observed. The regulatory T cells (Treg) factor, interleukin-10 (IL-10) and helper T cells 17(Th17) cytokines, interleukin-17A (IL-17A), as well as the Treg and Th17 cell were detected. Results Compared with the normal group, a significant rise of embryo loss rate presented in spontaneous abortion group (χ2=16.045, P<0.05); Compared with spontaneous abortion group, the embryo loss rate in VitD3 low dose group, middle dose group and high dose group reduced significantly (χ2=5.881, 13.704, 15.663, P<0.05). Compared with the normal group, the Treg, IL-10, Treg/Th17, IL-10/IL-17A in spontaneous abortion group reduced significantly; Th17 and IL-17A increased significantly, and the differences were statistically significant (P<0.05). Compared with spontaneous abortion group, the Treg, IL-10, Treg/Th17, IL-10/IL-17A in VitD3low dose group, middle dose group and high dose group increased significantly, while the Th17 and IL-17A decreased significantly, and they all had significantly difference based on statistical analysis (P<0.05).Conclusion VitD3 can reduce the embryo loss rate in the mouse model of spontaneous abortion, which may be related to adjusting the balance of Th17/Treg.

Key words: Vitamin D3, Spontaneous abortion, Embryo loss, Regulatory T cell/Helper T cell 17, Flow cytometry, Mouse