解剖学报

解剖学报 ›› 2017, Vol. 48 ›› Issue (5): 532-537.doi: 10.16098/j.issn.0529-1356.2017.05.006

• 神经生物学 • 上一篇    下一篇

医用臭氧通过下调神经病理性痛大鼠核因子κB/核因子κB抑制蛋白α/核因子κB抑制蛋白激酶β的表达发挥镇痛效应

马旭保1 上官军发2* 孙建民3   

  1. 1.吴忠市人民医院康复医学科，宁夏 吴忠 751100; 2.吴忠市人民医院神经外科，宁夏 吴忠 751100; 3.宁夏第三人民医院疼痛科， 银川 750021
  • 收稿日期:2016-10-25 修回日期:2017-01-04 出版日期:2017-10-06 发布日期:2017-10-06
  • 通讯作者: 上官军发 E-mail:maxubaowz@163.com

Ozone attenuated neuropathic pain induced by chronic constriction injury via suppression the expression of nuclear factor κB/inhibitor of  nuclear factor κBα/inhibitor of nuclear factor κB kinase β

MA Xu-bao1 SHANGGUAN Jun-fa2* SUN Jian-min3   

  1. 1.Department of Rehabilitation Medicine, Wuzhong People’s Hospital, Ningxia Wuzhong 751100, China; 2.Department of Neurosurgery, Wuzhong People’s Hospital, Ningxia Wuzhong 751100, China; 3.Department of Pain Treatment, The Third People’s Hospital of Ningxia, Yinchuan 750021, China
  • Received:2016-10-25 Revised:2017-01-04 Online:2017-10-06 Published:2017-10-06
  • Contact: SHANGGUAN Jun-fa E-mail:maxubaowz@163.com

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摘要：

目的 观察医用臭氧（OZ）对坐骨神经慢性缩窄性损伤（CCI）致神经病理性痛大鼠的镇痛作用及对核因子κB（NF-κB）、核因子κB抑制蛋白α（IκBα）及核因子κB抑制蛋白激酶β（IKKβ）表达水平的影响。 方法 采用CCI法复制大鼠神经病理性痛动物模型，同时给予不同剂量（0.8、0.4、0.2ml）的OZ予以干预，用Von Frey 纤维丝机械刺激触痛仪及冷板测痛仪测定不同剂量OZ对CCI大鼠的机械缩足反射阈值与冷缩足反射阈值的影响；用RT-PCR法和Western blotting法检测不同剂量OZ对CCI大鼠脊髓组织NF-κB p65、IκBα及IKKβ mRNA和蛋白表达水平的影响。 结果 与CCI神经病理性痛模型组比较，OZ 0.8、0.4ml剂量升高CCI大鼠机械缩足反射阈值，降低冷缩足反射阈值（P<0.05，P<0.01）；OZ 0.8、0.4ml剂量可下调CCI大鼠脊髓组织NF-κB p65、IκBα及IKKβ mRNA和蛋白表达水平（P<0.05，P<0.01）。 结论 OZ对CCI致神经病理性痛大鼠有镇痛作用，其机制可能与下调NF-κB p65、IκBα及IKKβ的表达有关。

关键词: 臭氧, 神经病理性痛, 核因子κB, 核因子κB抑制蛋白α, 核因子κB抑制蛋白激酶β, 免疫印迹法, 大鼠

Abstract:

Objective To investigate the analgesic effects of ozone treatment on expression of nuclear factor κB(NF-κB)/inhibitor of nuclear factor κBα(IκBα)/inhibitor of nuclear factor κB kinase β(IKKβ) in spinal cord tissue of rats with neuropathic pain induced by chronic constriction of sciatic nerve injury (CCI). Methods The neuropathic pain rats’ model was established by chronic constriction of sciatic nerve injury. Using the Von Fery Filaments system and cold plate to test the effect of ozone on mechanical withdrawal threshold (MWT) and cold withdrawal threshold (CWT), respectively; using the RT-PCT and Western blotting method to analysis the expressions of NF-κB, IκBα, IKKβ mRNA and protein in spinal cord tissue of CCI rats. Results Compared with the CCI model group rats, the ozone (0.8, 0.4 ml) could increase the CCI rats’ MWT, and decrease the CWT (P<0.05, P<0.01); the ozone (0.8, 0.4 ml) could down-regulate the CCI rats’ spinal cord tissue expression levels of NF-κB, IκBα and IKKβ mRNA and protein (P<0.05, P<0.01). Conclusion Ozone has analgesic effects on neuropathic pain induced by CCI, and its mechanism may be related to down-regulation of the NF-κB, IκBα and IKKβ.

Key words: Ozone, Neuropathic pain, Nuclear factor κB, Inhibitor of NF-κB α, nhibitor of nuclear factor κB kinase β, Western blotting, Rat