组织蛋白酶D在小鼠肺腺癌发生发展中的表达特点及其意义

doi:10.16098/j.issn.0529-1356.2018.01.009

解剖学报 ›› 2018, Vol. 49 ›› Issue (1): 56-62.doi: 10.16098/j.issn.0529-1356.2018.01.009

组织蛋白酶D在小鼠肺腺癌发生发展中的表达特点及其意义

张慧娟¹ 郑晓伟^1,2 乔玲¹常文静¹ 卢锋^1* 贾彩云^1*

1. 河南大学抗体药物开发技术国家地方联合工程实验室，河南开封 475004;2. 河南省濮阳市中医院检验科，河南濮阳 457000

收稿日期:2017-04-28 修回日期:2017-07-14 出版日期:2017-02-06 发布日期:2018-02-06
通讯作者: 卢锋1；贾彩云 E-mail:jcy2668@163.com
基金资助:
Cathepsin D新的糖基化异构体在肺癌发生发展中的作用及分子机制

Expression characteristics and significance of cathepsin D in the initiation and progression of mouse lung adenocarcinoma

ZHANG Hui-juan¹ ZHENG Xiao-wei ^1,2 QIAO Ling¹ CHANG Wen-jing¹ LU Feng ^1* JIA Cai-yun ^1*

1.He’nan University Joint National Laboratory for Antibody Drug Engineering, He’nan Kaifeng 475004, China; 2.Department of Clinical Laboratory, Puyang Hospital of Traditional Chinese Medicine, He’nan Puyang 475000, China

Received:2017-04-28 Revised:2017-07-14 Online:2017-02-06 Published:2018-02-06
Contact: LU Feng 1；JIA Cai-yun E-mail:jcy2668@163.com

摘要/Abstract

摘要：

目的探索组织蛋白酶D（CTSD）在肺腺癌发生发展中的表达特点及其意义。方法使用氨基甲酸乙酯腹腔注射建立BALB/c小鼠肺腺癌模型；蛋白质组放射性核素相对标记和绝对定量（iTRAQ）分析筛选差异表达蛋白；免疫组织化学和Western blotting检测蛋白在肺腺癌不同病理时期的表达变化特点。结果 CTSD蛋白在肺腺癌早期表达较对照组明显增强；免疫组织化学和Western blotting检验分析发现，随着肺腺癌病程进展，CTD表达呈上升趋势，在肺腺癌晚期，实验组为对照组13.7倍；出现肝转移实验组小鼠肺组织CTSD呈现不同异构体。结论 CTSD是肺组织癌变过程中的重要蛋白，其异常表达和修饰可能在肺腺癌发生、发展和转移中起重要作用，可能成为肺腺癌早期诊断的分子指标。

关键词: 组织蛋白酶D, 肺腺癌模型, iTRAQ定量分析, 免疫组织化学, 小鼠

Abstract:

Objective To investigate the expression characteristics and significance of cathepsin D(CTSD) in the initiation and progression of mouse lung adenocarcinoma.Methods We used urethane intraperitoneal injection to establish lung adenocarcinoma model in the BALB/c mouse. Differentially expressed proteins were identified using isobaric tag for relative and absolute quantitation(iTRAQ)-based quantitative proteomic technology in tumor group compared with control group. Cathepsin D expression characteristics were detected in different time points by immunohistochemical and Western blotting analysis. Results The expression of cathepsin D was significantly increased in the early-stage lung adenocarcinoma tissue compared with the control group. Immonohistochemical and Western blotting analysis observed that following tumor progression, cathepsin D expression was up-regulated. At the late-stage of lung adenocarcinoma, cathepsin D expression was 13.7-fold significantly higher than that in the control group and showed different molecular weight isoform of cathepsin D in the lung tissue of mouse with liver metastasis. Conclusion Cathepsin D is a critical protein in lung carcinogenesis. Aberrant expression and modification of cathepsin D may play an important role in the occurrence, development and metastasis of lung adenocarcinoma. Cathepsin D may have the potential to become biomarkers for early diagnosis of lung adenocarcinoma.

Key words: CathepsinD, Lung adenocarcinoma model, iTRAQ-based quantitative analysis, Immunohistochemistry, Mouse

张慧娟郑晓伟乔玲常文静卢锋贾彩云. 组织蛋白酶D在小鼠肺腺癌发生发展中的表达特点及其意义[J]. 解剖学报, 2018, 49(1): 56-62.

ZHANG Hui-juan ZHENG Xiao-wei QIAO Ling CHANG Wen-jing LU Feng JIA Cai-yun. Expression characteristics and significance of cathepsin D in the initiation and progression of mouse lung adenocarcinoma[J]. Acta Anatomica Sinica, 2018, 49(1): 56-62.

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组织蛋白酶D在小鼠肺腺癌发生发展中的表达特点及其意义

Expression characteristics and significance of cathepsin D in the initiation and progression of mouse lung adenocarcinoma

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