解剖学报

解剖学报 ›› 2018, Vol. 49 ›› Issue (2): 227-232.doi: 10.16098/j.issn.0529-1356.2018.02.015

• 组织学胚胎学发育生物学 • 上一篇    下一篇

高脂饮食诱导肥胖小鼠表达卵泡抑素样蛋白1与脂肪组织炎症和棕色脂肪的功能

王雪朦 房东亮 张迎 王璐璐 杨春 高艳*   

  1. 首都医科大学人体解剖学与组织学胚胎学系，北京 100069
  • 收稿日期:2017-11-27 修回日期:2018-01-08 出版日期:2017-04-06 发布日期:2018-04-06
  • 通讯作者: 高艳 E-mail:gy1003@ccmu.edu.cn
  • 基金资助:
    国家自然科学基金;北京市自然科学基金;北京市教育委员会科技计划面上项目;北京市优秀人才青年骨干个人项目;“十三五”时期北京市属高校高水平教师队伍建设支持计划

Expression of follistatinlike protein 1, adipose tissue inflammation and brown fat in obese mice induced obesity by high-fat diet

WANG Xue-meng FANG Dong-liang ZHANG Ying WANG Lu-lu YANG Chun GAO Yan*   

  1. Department of Anatomy, Histology and Embryology, Capital Medical University, Beijing 100069, China
  • Received:2017-11-27 Revised:2018-01-08 Online:2017-04-06 Published:2018-04-06
  • Contact: GAO Yan E-mail:gy1003@ccmu.edu.cn

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摘要：

目的 探讨卵泡抑素样蛋白1（FSTL1）在高脂饮食诱导的肥胖小鼠脂肪炎症中的作用，以及对棕色脂肪功能的影响。 方法 6周龄C57BL/6 J小鼠分为两组，每组8只，给予12周正常饮食（RD）和高脂饮食（HFD）饲养，每周称量小鼠体重，记录小鼠摄食量；12周后，进行糖耐量和胰岛素耐量的检测并取材，称量小鼠的皮下白色脂肪、肾周白色脂肪、附睾白色脂肪和棕色脂肪的重量；HE染色观察小鼠白色脂肪中的冠状结构（CLS）及小鼠棕色脂肪组织变化，RT-PCR分析小鼠附睾白色脂肪炎症因子、肿瘤坏死因子α（TNF-α)、白细胞介素6（IL-6）和白细胞介素10（IL-10）的表达，以及FSTL1的表达；Western blotting检测棕色脂肪解耦联蛋白1（UCP1）与FSTL1蛋白的表达。 结果 与RD小鼠相比，HFD组小鼠的体重增加，摄食量减少，不同部位脂肪重量显著增加，葡萄糖耐受能力显著降低，胰岛素敏感性显著下降，附睾白色脂肪观察到炎症反应CLS且附睾白色脂肪中炎症因子TNF-α、IL-6及IL-10表达显著增高， FSTL1表达亦显著增加。Western blotting结果显示，HFD小鼠的棕色脂肪组织中UCP1与FSTL1表达水平均显著性高于RD组。 结论 在高脂饮食诱导的肥胖小鼠中，FSTL1的表达可能与脂肪炎症因子的分泌增加及棕色脂肪的功能有一定相关性。

关键词: 卵泡抑素样蛋白1, 炎症因子, 棕色脂肪组织, HE染色, 免疫印迹法, 小鼠

Abstract:

Objective To investigate the effect of follistatin-like protein 1 (FSTL1) on inflammation and brown adipose tissue (BAT) in high-fat diet induced obesity in C57BL/6 J mice. Methods C57BL/6 J mice were divided into two groups and every group contained eight mice. Mice at 6-week of age were fed with high fat diet (HFD) or regular diet (RD) for 12 weeks. The body weight of the mice was measured every week, and the food intake was also recorded. After 12 weeks, glucose tolerance test and insulin tolerance test were performed before sacrifice. Subcutaneous white adipose tissue (SWAT), perirenal white adipose tissue (PWAT), epididymis white adipose tissue (EWAT), and brown adipose tissue (BAT) were collected and weighed. HE staining was applied toobserve Crown-like structures (CLS) in SWAT, PWAT, EWAT and the change of BAT. RT-PCR was performed to evaluate the mRNA levels of tumor necrosis factor (TNF-α), interleukin(IL)-6, IL-10 and FSTL in EWAT. Western blotting was used to analyze the protein levels of uncoupling protein 1(UCP1) and FSTL1 in BAT.Results The data showed that body weight and fat weight were significantly increased in HFD mice compared to those in RD mice. Food intake were significantly decreased in HFD mice compared to that in RD mice. Impaired glucose tolerance and insulin tolerance were present in HFD mice. In addition, CLS were observed in EWAT, but not in SWAT and PWAT of HFD mice. The mRNA levels of TNF-α, IL-6 and IL-10 in EWAT from HFD mice were significantly increased compared to those from RD mice. Meanwhile, The mRNA levels of FSTL1 in EWAT from HFD mice were also significantly increased compared to that from RD mice. The data from Western blotting showed that both UCP1 and FSTL1 were significantly increased in BAT of HFD mice compared to those in RD mice. Conclusion The present data indicates that the increased expression of inflammation-related genes and function of BAT may be related to the high level of FSTL1 in HFD-induced obesity.

Key words: Follistatin-like protein 1, Inflammatory factor, Brown adipose tissue, HE staining, Western blotting, Mouse