解剖学报

›› 2007, Vol. 38 ›› Issue (5): 625-627.doi:

• 新技术方法 • 上一篇    下一篇

酶标荧光-97检测中性粒细胞碱性磷酸酶活性的新方法

邸菁1; 张凌志2; 柏树令1*; 赵海花1   

  1. 1 中国医科大学基础医学院人体解剖学教研室，沈阳 110001; 2 中国医科大学盛京医院急诊科，沈阳110004
  • 收稿日期:2007-01-11 修回日期:2007-04-03 出版日期:2007-10-06
  • 通讯作者: 柏树令

ENZYME LABELED FLUORESCENCE 97 TO DETECT THE ACTIVITY OF ALKALINE PHOSPHATASE IN NEUTROPHILS

  1. 1. Department of Anatomy, China Medical University, Shenyang 110001, China;2. Department of Emergency, Shengjing Hospital Affiliated, China Medical University, Shenyang 110004, China
  • Received:2007-01-11 Revised:2007-04-03 Online:2007-10-06
  • Contact: BAI Shu-ling

关键词: 酶标荧光-97, 中性粒细胞, 碱性磷酸酶, 酶细胞化学, 荧光/微分干涉相差显微镜, 人

Abstract: Objective To localize endogenous alkaline phesphatase in human neutrophils with ELF (enzyme-labeled fluorescence)-97 phosphate, which yields an intensely fluorescent yellow-green precipitate at the site of enzymatic activity. Methods Neutrophils were isolated from human blood and fixed,then histochemistry with the ELF-97 phosphate was performed with the ELF-97 endogenous phosphatase detection kit.All photography was performed with a Nikon labophot fluorescence/DIC microscope. Results Fluorescent yellow-green granules, well-distributed but size-varied, were observed in neutrophils under fluorescence microscope.There were 94.102±3.133 percent rate of positive neutrophil alkaline phosphatase among 51 cases of healthy volunteers.Conclusion ELF-97 endogenous phosphatase detection kit can provide sensitive,high-resolution localization of endogenous phosphatase activity in neutrophils.

Key words: ELF-97, Neutrophil, Alkaline phosphatase, Enzyme cytochemistry, Differential interference contrast microscope, Human

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