解剖学报

解剖学报 ›› 2019, Vol. 50 ›› Issue (6): 722-728.doi: 10.16098/j.issn.0529-1356.2019.06.004

• 神经生物学 • 上一篇    下一篇

迷走神经途径的L-谷氨酸与十二指肠运动的量效关系

张金1 张莹利1 李想2 杜宜楠1 赵建帅1 张柳凝2 徐永平1*   

  1. 1.西北农林科技大学动物医学院解剖学与组织学胚胎学教研室，陕西 杨凌 712100;2.西北农林科技大学生命科学学院生物科学教研室，陕西 杨凌 712100
  • 收稿日期:2018-11-13 修回日期:2018-12-24 出版日期:2019-12-06 发布日期:2019-12-06
  • 通讯作者: 徐永平 E-mail:xuyp717@nwsuaf.edu.cn
  • 基金资助:
    陕西省农业推广项目

Dose-effect relationship between L-glutamic acid and duodenal movement in the vagus pathway

ZHANG Jin1 ZHANG Ying-li1 LI Xiang2 DU Yi-nan1 ZHAO Jian-shuai1 ZHANG Liu-ning2 XU Yong-ping 1*   

  1. 1.Department of Anatomy and Histology and Embryology，College of Veterinary Medicine, Northwest A&F University，Shanxi Yangling 712100,China; 2.Department of Biological Science，College of Life Siences，Northwest A&F University， Shanxi Yangling 712100,China
  • Received:2018-11-13 Revised:2018-12-24 Online:2019-12-06 Published:2019-12-06
  • Contact: XU Yong-ping E-mail:xuyp717@nwsuaf.edu.cn

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摘要：

目的 构建经迷走神经背核（DMV）注射L-谷氨酸(L-Glu)的剂量与雌性家兔十二指肠肌电活动指数的数学模型，为探讨激素、细胞因子等影响迷走神经活动的作用机制提供实验依据。 方法 采用DMV显微注射技术，分别按0 mol/L、0.05 mol/L、0.10 mol/L、0.15 mol/L和0.20 mol/L剂量经DMV注射L-Glu，每剂量组样本数为5只，监测卵巢摘除家兔的十二指肠肌电活动；并构建L-Glu的剂量与十二指肠肌电活动指数之间的数学模型。 结果 经t检验分析，相邻剂量组之间，肌电活动的振幅逐渐增强，且差异极显著（P＜0.01）；频率逐渐加强，其中0 mol/L与0.05 mol/L、0.10 mol/L与0.15 mol/L组间差异有显著性（P＜0.05）；肌电活动指数逐渐增强，且组间差异极显著（P＜0.01）。以LGlu浓度为自变量 x，十二指肠肌电活动指数为因变量 y，构建的数学模型的函数关系式为：y=13.71/1 + exp ［-22.35 × (x -0.082)］，函数拟合精度：R2=0.9948，P<0.01，属于增长型S型逻辑函数。函数分析表明，0.082 mol/L L-Glu剂量为该逻辑函数的拐点值；在L-Glu剂量范围为0 mol/L～0.082 mol/L时，十二指肠肌电活动指数成指数型增长模式；当L-Glu剂量大于0.082 mol/L时，十二指肠肌电活动指数呈对数型增长模式，且其理论极限值为13.71。 结论 L-Glu通过DMV对家兔十二指肠肌电活动的促进作用具有显著的量效关系，且具有区间作用的特点。

关键词: L-谷氨酸, 迷走神经背核, 肌电活动指数, 数学模型, 十二指肠, 脑立体定位法, 雌性家兔

Abstract:

Objective To establish a mathematical model of the injection by dorsal motor nucleus of vagus(DMV), the dose of L-glutamic acid（L-Glu）and the duodenal myoelectric activity index of female rabbits was established to provide a theoretical basis for studying the mechanism of action of hormones, cytokines and other vagal activity. Methods In this experiment, we used microinjection technique to inject L-Glu to DMV by doses of 0 mol/L, 0.05 mol/L, 0.10 mol/L, 0.15 mol/L and 0.20 mol/L,monitoring the duodenal myoelectric activity of ovarian abducted rabbits,the number of samples per dose group was 5, and construct a mathematical model between the dose of L-Glu and the index of duodenal myoelectric activity. Results After t-test analysis, the amplitude of myoelectric activity increased gradually between adjacent dose groups, and the difference was extremely significant (P<0.01).The frequency was gradually strengthened, and the difference between 0 mol/L and 0.05 mol/L, 0.10 mol/L and 0.15 mol/L was significant (P<0.05).The index of myoelectric activity increased gradually, and the difference between groups was extremely significant (P<0.01). With L-Glu concentration as the independent variable x, the duodenal myoelectric activity index was the dependent variable y, the constructor relationship was y=13.71/1 + exp ［-22.35 × (x-0.082)］, function fitting accuracy was R2=0.9948，P<0.01，belonging to growth type S logic function. Function analysis showed that the L-Glu dose of 0.082 mol/L was the inflection point of the logic function. As the dose range of L-Glu was 0 mol/L 0.082 mol/L,duodenal myoelectric activity index showed an exponential growth pattern,as the L-Glu dose was greater than 0.082 mol/L,the duodenal myoelectric activity index showed a logarithmic growth pattern with a theoretical limit of 13.71. Conclusion L-Glu has a significant dose-effect relationship on the promotion of duodenal myoelectric activity in rabbits by DMV, and have the effect of interval,the mathematical model laid the theoretical foundation for further research on the role of hormones and cytokines on this basis.

Key words:  L-glutamic acid, Dorsal motor nucleus of the vagus, Myoelectric activity index, Mathematical model, Duodenum, Stereotactic method of brain, Female rabbit