藤黄酸通过核因子κB抑制剂激酶α/p65信号通路抑制人胃癌细胞系SGC-7901的侵袭

doi:10.16098/j.issn.0529-1356.2020.01.008

解剖学报 ›› 2020, Vol. 51 ›› Issue (1): 47-50.doi: 10.16098/j.issn.0529-1356.2020.01.008

藤黄酸通过核因子κB抑制剂激酶α/p65信号通路抑制人胃癌细胞系SGC-7901的侵袭

仝雷¹王世广²杨占峰³岳丽晓¹ 张玲玲¹王鹏^2*

1.郑州工业应用技术学院医学形态学教研室，河南新郑 451150; 2.郑州工业应用技术学院医学机能学教研室，河南新郑 451150; 3.郑州工业应用技术学院预防医学教研室，河南新郑 451150

收稿日期:2018-12-12 修回日期:2019-11-02 出版日期:2020-02-06 发布日期:2020-04-21
通讯作者: 王鹏 E-mail:upliz@zzu.edu.cn
基金资助:
河南省高等学校重点科研项目;河南省卫生与计划生育委员会医学教育研究课题

Gambogic acid suppresses invasion in human gastric carcinoma SGC-7901 cells by modulating inhibitor of nuclear factor kappa-B kinase α/p65 signaling pathway in vitro#br#

TONG Lei¹ WANG Shi-guang² YANG Zhan-feng³ YUE Li-xiao¹ZHANG Ling-ling¹WANG Peng^2*#br#

1.Department of Medical Morphology, Zhengzhou University of Industrial Technology, He’nan Xinzheng 451150, China; 
2.Department of Medical Function, Zhengzhou University of Industrial Technology, He’nan Xinzheng 451150, China; 
3.Department of Phylaxiology, Zhengzhou University of Industrial Technology, He’nan Xinzheng 451150, China

Received:2018-12-12 Revised:2019-11-02 Online:2020-02-06 Published:2020-04-21
Contact: WANG Peng E-mail:upliz@zzu.edu.cn

摘要/Abstract

摘要：

目的探讨藤黄酸（GA）对人胃癌SGC-7901细胞侵袭能力的影响及其机制。方法用不同浓度藤黄酸、核因子κB抑制剂激酶（IKK16）和阳性对照药物5-氟尿嘧啶作用人正常胃黏膜上皮GES-1细胞和人胃癌SGC-7901细胞48 h后，采用细胞计数试剂盒8（CCK-8）检测细胞活力，Transwell侵袭实验检测SGC-7901细胞的侵袭能力，免疫印迹法检测波形蛋白（vimentin）、基质金属蛋白酶2（MMP-2）和基质金属蛋白酶9（MMP-9）的蛋白表达水平以及IKKα和p65的蛋白磷酸化水平。结果藤黄酸可剂量依赖性地抑制SGC-7901细胞活力，半数抑制浓度（IC₅₀）分别为1.89 μmol/L，但对GES-1细胞活力无显著影响；5-氟尿嘧啶对GES-1和SGC-7901细胞均有抑制作用，IC50 分别为7.36 μmol/L和199.57 μmol/L。低剂量藤黄酸或IKK16可抑制SGC-7901细胞侵袭，下调MMP-2、MMP-9和vimentin的蛋白表达水平以及抑制IKKα和p65蛋白磷酸化，并且两者联合作用时抑制作用更强。

结论低剂量藤黄酸可在体外抑制人胃癌SGC-7901细胞侵袭，其机制可能与抑制IKKα/p65信号通路，继而下调MMP-2、MMP-9和vimentin蛋白表达水平相关。

关键词: 藤黄酸, 胃癌, 侵袭, SGC-7901细胞, 核因子κB抑制激酶α, 免疫印迹法, 人

Abstract:

Objective To investigate the effect of gambogic acid (GA) on invasion in human gastric carcinoma SGC-7901 cells and its possible mechanism. Methods Cell counting kit-8(CCK-8) assay was performed to detect the effects of GA, inhibitor of nuclear factor kappa-B kinase(IKK)16 and 5-fluorouracil (5-FU) on cell activity of GES-1 and SGC-7901 cells. Cell invasion was assessed with Transwell invasion assay. Western blotting was used to analyze the protein levels of vimentin, matrix metalloproteinase 2 (MMP-2) and MMP-9 and protein phosphorylation of IKKα and p65. Results The cell activity was significantly decreased in SGC-7901 cells treated with GA in a dose-dependent manner with a half inhibiton concentration(IC50) value of 1.89 μmol/L. But GA had no significant influence on cell viability of GES-1 cells. Meanwhile, 5-FU reduced the cell activity of GES-1 and SGC-7901 cells with IC50 values of 7.36 μmol/L and 199.57 μmol/L respectively. Low-dose GA and IKK16 impaired separately the ability of invasion in SGC-7901 cells, and down-regulated the protein levels of MMP-2, MMP-9 and vimentin, and inhibited phosphorylation of IKKα and p65, while a stronger inhibition was showed when the combination of GA and IKK16 was used. Conclusion Low-dose GA might inhibit invasion of SGC-7901 cells via IKKα/p65 signaling pathway.

Key words: Gambogic acid, Gastric carcinoma, Nvasion, SGC-7901 cells, Inhibitor of nuclear factor kappa-B kinaseα, Western blotting, Human

中图分类号:

R735.2

仝雷王世广杨占峰岳丽晓张玲玲王鹏. 藤黄酸通过核因子κB抑制剂激酶α/p65信号通路抑制人胃癌细胞系SGC-7901的侵袭[J]. 解剖学报, 2020, 51(1): 47-50.

TONG Lei WANG Shi-guang YANG Zhan-feng YUE Li-xiao ZHANG Ling-ling WANG Peng. Gambogic acid suppresses invasion in human gastric carcinoma SGC-7901 cells by modulating inhibitor of nuclear factor kappa-B kinase α/p65 signaling pathway in vitro#br#[J]. Acta Anatomica Sinica, 2020, 51(1): 47-50.

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藤黄酸通过核因子κB抑制剂激酶α/p65信号通路抑制人胃癌细胞系SGC-7901的侵袭

Gambogic acid suppresses invasion in human gastric carcinoma SGC-7901 cells by modulating inhibitor of nuclear factor kappa-B kinase α/p65 signaling pathway in vitro#br#

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