解剖学报

解剖学报 ›› 2021, Vol. 52 ›› Issue (5): 712-719.doi: 10.16098/j.issn.0529-1356.2021.05.007

• 细胞和分子生物学 • 上一篇    下一篇

大鼠成纤维细胞生长因子对心肌成纤维细胞增殖和转分化的作用机制

刘梦昕1 孙昌业1 李铎1 任明芬2 郭志坤1*
  

  1. 1. 新乡医学院 河南省医用组织再生重点实验室; 2. 新乡医学院第二附属医院心血管内科，河南 新乡 453003
  • 收稿日期:2020-05-26 修回日期:2020-08-12 出版日期:2021-10-06 发布日期:2021-10-06
  • 通讯作者: 郭志坤 E-mail:gzk@xxmu.edu.cn
  • 基金资助:
    国家自然科学基金项目;新乡医学院研究生科研创新支持计划项目

Effect and mechanism of fibroblast growth factor on proliferation and transdifferentiation of cardiac fibroblasts in rats#br#

LIU Meng-xin1 SUN Chang-ye1 Li Duo1 REN Ming-fen2 GUO ZhI-kun1*#br#   

  1. 1.He’nan Key Laboratory of Medical Tissue Regeneration of Xinxiang Medical University, He’nan Xinxiang 453003, China;2.Department of Cardiology, the Second Affiliated Hospital of Xinxiang Medical University,  He’nan Xinxiang 453003, China
  • Received:2020-05-26 Revised:2020-08-12 Online:2021-10-06 Published:2021-10-06
  • Contact: GUO ZhI-kun E-mail:gzk@xxmu.edu.cn
  • Supported by:
    National Natural Science Foundation of China

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摘要：

目的 探讨成纤维细胞生长因子（FGF）对心肌成纤维细胞(CFs)在增殖和转分化为肌成纤维细胞（MFs）中的作用。   方法  分离、培养大鼠CFs，利用FGF对其进行诱导培养，CCK-8技术检测细胞活性和增殖状况，免疫荧光和Western blotting技术测定α平滑肌肌动蛋白（α-SMA）和Ⅰ型胶原（ColⅠ）的表达量。   结果  随大鼠CFs培养代数的增加，其α-SMA、ColⅠ的表达和活化为MFs的数量增加。加入FGF诱导培养的大鼠CFs数量没有明显增加；加入FGF1、FGF2诱导的CFs表达α-SMA减少，活化为MFs 数量减少。   结论  FGF家族对大鼠CFs没有促增殖作用，但FGF1和FGF2可以抑制CFs活化，减少其向MFs的分化。

关键词: 成纤维细胞生长因子, α平滑肌肌动蛋白, 肌成纤维细胞, 心肌成纤维细胞, 免疫印迹法, 大鼠

Abstract:

Objective  To investigate the effect of fibroblast growth factor (FGF) on the proliferation and transdifferentiation of cardiac fibroblasts (CFs) into myofibroblasts (MFs).    Methods  Rat CFs were isolated and cultured, and then induced by FGF. CCK-8 was used to detect the cell activity and proliferation. Immunofluorescence and Western blotting were used to detect the expression of α smooth muscle actin (α-SMA) and collagen Ⅰ (ColⅠ).    Results  The expression and activation of α-SMA and ColⅠ increased with the increase of CFs culture generation. The number of CFs induced by FGF did not increased significantly; the expression of α-SMA in CFs induced by FGF1 and FGF2 decreased, and the number of activated MFs decreased.    Conclusion  FGF family has no effect on the proliferation of CFs, but FGF1 and FGF2 can inhibit the activation of CFs and reduce the differentiation into MFs.

Key words: Fibroblast growth factor, α-Smooth muscle actin, Myofibroblast, Cardiac fibroblast, Western blotting, Rat

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