基于癌基因组图谱数据挖掘分析CC亚族趋化因子配体20在食管癌中的表达及临床意义

doi:10.16098/j.issn.0529-1356.2022.01.008

解剖学报 ›› 2022, Vol. 53 ›› Issue (1): 60-65.doi: 10.16098/j.issn.0529-1356.2022.01.008

基于癌基因组图谱数据挖掘分析CC亚族趋化因子配体20在食管癌中的表达及临床意义

秦波¹ 王正洋²殷凡响¹闫丹¹朱珠^3*

1.郑州大学第一附属医院转化医学中心，郑州 450052; 2.郑州大学第一附属医院病理科，郑州 450052; 3.郑州大学第一附属医院生物样本库，郑州 450052

收稿日期:2020-09-25 修回日期:2020-12-22 出版日期:2022-02-06 发布日期:2022-02-06
通讯作者: 朱珠 E-mail:zzjy86@163.com
基金资助:
国家自然科学基金青年项目;国家自然科学基金青年项目; 河南省重点研发与推广专项

Expression and clinical significance of CC motif chemokine ligand 20 in esophageal carcinoma based on the Cancer Genome Atlas data mining

QIN Bo¹ WANG Zheng-yang² YIN Fan-xiang¹ YAN Dan¹ ZHU Zhu^3*

1.Department of Translational Medicine Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; 2.Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; 3.Department of Biological Sample Bank, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China

Received:2020-09-25 Revised:2020-12-22 Online:2022-02-06 Published:2022-02-06
Contact: ZHU Zhu E-mail:zzjy86@163.com
Supported by:
National Natural Science Foundation of China;National Natural Science Foundation of China

摘要/Abstract

摘要：

目的探讨CC亚族趋化因子配体20（CCL20）在食管癌中的表达及对病程进展和预后的影响。方法利用癌基因组图谱 (TCGA)、UALCAN、GEO等相关数据库分析CCL20基因在食管癌组织与癌旁组织中的表达差异，通过Kaplan-Meier模型探讨CCL20表达水平与预后的关联性；分析CCL20与肥胖、甲基化等之间的关系；最后通过mRNA、蛋白水平，分析CCL20在食管癌组织和癌旁组织的表达差异。结果 CCL20在食管癌尤其是腺癌类型中高表达，高表达CCL20患者生存率明显降低；CCL20的表达量与食管癌患者肥胖程度成正比、与其甲基化程度成反比；食管癌组织中CCL20的表达量显著增加。结论 CCL20在食管癌组织中高表达，且CCL20高表达与患者的生存预后成显著负相关。

关键词: 食管癌, CC亚族趋化因子配体20, 癌基因组图谱数据库, 预后, 免疫组织化学, 人

Abstract:

Objective To explore the expression and significance of CC mokif chemokine ligand 20(CCL20) gene in esophageal carcinoma and its influence on the progression and prognosis of the disease. Methods The expression data of esophageal cancer were obtained from the Cancer Genome Atlas (TCGA) database, and were collected from cBioPortal(www.cbioportal.org).The survival curve was analyzed by Kaplan-Meier method . Through the website (http://ualcan.path.uab.edu) the objective was to analyze the expression of CCL20 gene in esophageal cancer tissues, the relationship between CCL20 and obesity and the methylation level of CCL20 in esophageal cancer. cBioPortal was used to analyze the copy number and mRNA expression of CCL20 gene. Finally, the expression differences of CCL20 in esophageal cancer tissues and adjacent tissues were analyzed by mRNA and protein levels. Results CCL20 was highly expressed in esophageal cancer, especially adenocarcinoma. The survival rate of patients with high expression of CCL20 was reduced. The expression of CCL20 was directly proportional to the degree of obesity in patients with esophageal cancer and inversely proportional to the degree of methylation. The expression of CCL20 in esophageal cancer tissues increased significantly. Conclusion CCL20 gene expression level is associated with poor prognosis of esophageal cancer patients.

Key words:

Esophageal cancer, CC motif chemokine ligand 20, The Cancer Genome Atlas database, Prognosis, Immunohistochemistry, Human

中图分类号:

R735.1

秦波王正洋殷凡响闫丹朱珠. 基于癌基因组图谱数据挖掘分析CC亚族趋化因子配体20在食管癌中的表达及临床意义[J]. 解剖学报, 2022, 53(1): 60-65.

QIN Bo WANG Zheng-yang YIN Fan-xiang YAN Dan ZHU Zhu. Expression and clinical significance of CC motif chemokine ligand 20 in esophageal carcinoma based on the Cancer Genome Atlas data mining[J]. Acta Anatomica Sinica, 2022, 53(1): 60-65.

参考文献

［1］ Torre LA, Bray F, Siegel RL, et al. Global cancer statistics［J］.CA Cancer J Clin, 2012，65 (2):7-108.

［2］ Lordick F, Hlscher AH, Haustermans K, et al. Multimodal treatment of esophageal cancer［J］. Langenbecks Arch Surg, 2013, 398 (2):177-187.

［3］ Chen ZhJ, Liao ShY, Wu WL, et al. Expression of chemokines CCL20 and SDF-1 in colorectal cancer and their relationship with lymph node and liver metastasis［J］. Guangdong Medicine, 2018, 39(17):2598-2601，2605. (in Chinese)

陈志京, 廖山婴, 吴伍林, 等. 趋化因子CCL20、SDF-1在大肠癌中的表达及其与淋巴结及肝脏转移的关系［J］. 广东医学, 2018, 39(17):2598-2601, 2605.

［4］ Walch-Rückheim B, Mavrova R, Henning M, et al. Stromal Fibroblasts Induce CCL20 through IL6/C/EBPβ to support the recruitment of Th17 cells during cervical cancer progression［J］. Cancer Res, 2015,75(24):5248-5259.

［5］ Geismann C, Grohmann F, Dreher A, et al. Role of CCL20 mediated immune cell recruitment in NF-κB mediated TRAIL resistance of pancreatic cancer［J］. Biochim Biophys Acta Mol Cell Res, 2017, 1864(5):782-796.

［6］ Kadomoto S, Izumi K, Mizokami A. The CCL20-CCR6 axis in cancer progression ［J］. Int J Mol Sci, 2020, 21(15):5186.

［7］ Lee AYS, K?rner H. The CCR6-CCL20 axis in humoral immunity and T-B cell immunobiology［J］. Immunobiology, 2019, 224(3):449-454.

［8］ Xiao JL, Zheng X, Deng H, et al. Expression of CCR6 in esophagealsquamous cell carcinoma and its effects on epithelial-to-mesenchymal transition［J］. Oncotarget, 2017, 8(70): 115244-115253.

［9］ Lian J, Liu S, Yue Y, et al. Eomes promotes esophageal carcinoma progression by recruiting Treg cells through the CCL20-CCR6 pathway［J］. Cancer Sci, 2021, 112(1):144-154.

［10］ Wang Y, Lyu Zh, Qin Y, et al. FOXO1 promotes tumor progression by increased M2 macrophage infiltration in esophageal squamous cell carcinoma［J］. Theranostics, 2020,10(25):11535-11548.

［11］ Liu JY, Li F, Wang LP, et al. CTL-vs Treg lymphocyte-attracting chemokines, CCL4 and CCL20, are strong reciprocal predictive markers for survival of patients with oesophageal squamous cell carcinoma［J］. Br J Cancer, 2015,113(5): 747-755.

［12］ Lauby-Secretan B, Scoccianti Ch, Loomis D, et al. Body fatness and cancer——viewpoint of the IARC Working Group［J］. N Engl J Med, 2016, 375: 794-798.

［13］ Wunderlich CM, Ackermann PJ, Ostermann AL, et al. Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment［J］. Nat Commun, 2018, 9(1):1646.

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基于癌基因组图谱数据挖掘分析CC亚族趋化因子配体20在食管癌中的表达及临床意义

Expression and clinical significance of CC motif chemokine ligand 20 in esophageal carcinoma based on the Cancer Genome Atlas data mining

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