›› 2009, Vol. 40 ›› Issue (5): 766-769.doi: 10.3969/j.issn.0529-1356.2009.05.014

• 论著 • 上一篇    下一篇

雌激素改善卵巢切除合并慢性铝中毒对大鼠心脏Cx43表达和金属元素变化的影响

徐振平1;赵长安2 ;许娜3;张文4;郭志坤5*   

  1. 1. 新乡医学院细胞生物及遗传学教研室;2. 生物化学与分子生物学教研室;3.形态学实验室; 4.分析测试实验室; 5. 河南省高等学校组织再生重点实验室,新乡 453003
  • 收稿日期:2008-08-18 修回日期:2008-10-17 出版日期:2009-10-06
  • 通讯作者: 郭志坤5

P>Effect of estrogen replacement threapy on Cx43 expression and metal elements in ovariectomy and chronic aluminum toxication rat heart /P>

  1. 1.Department of Cell Biology and Genetics of Xinxiang Medical University;2. Department of Biochemistry and Molecular Biology; 3. Laboratory of Morphology;4. Laboratory of Analysis and Test;5.He′nan Key Laboratory for Tissue Regeneration,He′nan Xinxiang 453003, China
  • Received:2008-08-18 Revised:2008-10-17 Online:2009-10-06
  • Contact: GUO Zhi-kun

关键词: Cx43, 金属元素, 雌激素, 卵巢切除, 心脏, 免疫组织化学, 大鼠

Abstract: Objective To reveal the influence of estrogen replacement threapy on the gap junction connexin 43(Cx43)expression and content of metal elements in ovariectomy and chronic aluminum toxication rat heart. Methods Forty 6-month-old female SD rats were divided randomly into 4 groups: group Sham, group OVX, group OVX+Al and group OVX+Al+ESUB>2/SUB>. After 3 months, The content of metalelements in heart was detected by ICP-AES. The Cx43 expressions were observed and determinated by immunohistochemistry and digital image analysis. Results 1.Cx43 positive granules distributed in cell side surface and cytoplasm as well as intercalated disk in atrial myocytes. They typically confined to the site of intercalated disk in ventricular myocytes in group Sham. In Group OVX, the Cx43 of atrial myocytes were redistributed from cell side surface to the intercalated disk. They were redistributed from the intercalated disk to cell side surface and in cytoplasm in ventricular myocytes. And some Cx43 granules distruibuted irregularly. The Cx43 redistribution of group OVX+Al was more irregular than that of group OVX. In group OVX+Al+E2, the Cx43 distribution of atrial myocytes returned to normal. 2. The amount of Cx43 in all groups was not changed. 3. In sham group,the content of mental elements in heart showed as follows: Mg>Ca>Fe>Zn>Al>Si>Cu>Mn>Se and Cd; Compared between group OVX and group sham, the content of Zn decreased (EM>P/EM><0.001); Compared between group OVX+A1+ESUB>2/SUB> and group sham, the content of Al, Cd, Si and Se increased (P<0.05,EM>P/EM><0.01,EM>P/EM><0.01,EM>P/EM><0.001); Compared between group OVX+Al+ESUB>2/SUB> and group OVX+Al, the content of Cd, Mn and Se inc

Key words: Connexin 43, Metal element (ion), Estrogen, Ovariectomy, Heart, Immunohistochemistry, Rat

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