解剖学报

解剖学报 ›› 2016, Vol. ›› Issue (3): 381-385.doi: 10.16098/j.issn.0529-1356.2016.03.016

• 组织学胚胎学发育生物学 • 上一篇    下一篇

分泌性腹泻对小鼠钙激活Cl-通道基因表达的影响

李和平 关艳敬 查光明 汪新建 王月影*   

  1. 河南农业大学农业部动物生化与营养重点开放实验室， 郑州 450002
  • 收稿日期:2015-11-05 修回日期:2016-02-13 出版日期:2016-06-06 发布日期:2016-06-06
  • 通讯作者: 王月影 E-mail:liheping1972@126.com
  • 基金资助:

    农业部转基因重大专项;郑州市科技攻关计划

Effects of secretory diarrhea on calcium activated chloride channel gene expression in the mouse

LI He-ping GUAN Yan-jing ZHA Guang-ming WANG Xin-jian WANG Yue-ying*   

  1. Key Laboratory of Animal Chemical and Nutrition of Agricultural Ministry of China, He’nan Agricultural University,  Zhengzhou 450002, China
  • Received:2015-11-05 Revised:2016-02-13 Online:2016-06-06 Published:2016-06-06
  • Contact: WANG Yue-ying E-mail:liheping1972@126.com

摘要：

目的 探讨肠道组织钙激活Cl-通道（CLCAs）基因表达与分泌性腹泻发生的关系。 方法 选取昆明小鼠24只，雌雄各半，随机分为3组：对照组、实验1h和8h组，每组8只。对照组小鼠经腹腔注射0.2ml生理盐水，实验组小鼠经腹腔注射脂多糖（LPS，6mg/kg）分别作用1h和8h，于注射后观察小鼠活动特征及肠道组织形态，以判定分泌性腹泻模型的建立，利用实时光定量PCR法检测各段肠道组织CLCAs基因的表达。 结果 LPS成功诱导小鼠发生了分泌性腹泻；小鼠十二指肠、空肠、回肠和结肠中均有CLCAs基因的转录；注射LPS后，十二指肠、空肠、回肠和结肠的CLCA1、CLCA2、CLCA3、CLCA4、CLCA6基因转录水平均发生上调，与1h组相比，8h组各基因转录水平显著上调。注射LPS后，十二指肠未检测到CLCA5的表达，但空肠、回肠CLCA5的表达均上调。 结论 LPS引起的分泌性腹泻与各肠段CLCAs基因的差异表达有关。

Abstract:

Objective To investigate effects of secretory diarrhea on calcium activated chloride channel（CLCAs）gene expression in the mouse intestinal tissue. Methods Twenty-four Kunming mice (12 males and 12 females) were randomly divided into three groups (n=8 in each group): control, experimental 1 hour and 8 hours groups. The control group were given a normal saline intraperitoneal injection of 0.2 ml, the experimental group were treated an by intraperitoneal injection of lipopolysaccharide (LPS，6 mg/kg) for 1hour or 8hours respectively. After injection for 1 hour or 8 hour, the mouse mental state and intestinal morphology were observed to evaluating secretory diarrhea model establishment. Real-time PCR was used to detect CLCAs gene expression in the intestine. Results The results showed that, LPS induced secretory diarrhea in mice, CLCAs genes were expressed in several intestine segments. After LPS injection, the transcriptional levels of CLCA1, CLCA2, CLCA3, CLCA4, CLCA6 gene in duodenum, jejunum, ileum and colon were up-regulated. Compared with 1 hour group, the gene transcription level significantly up-regulated in 8 hours group. After LPS injection, CLCA5 expression was not detected in duodenum, but expression of CLCA5 was significantly up-regulated in jejunum and ileum. Conclusion The different CLCAs gene expression in intestinal segments is related to secretory diarrhea caused by LPS.