关键词: 宫颈上皮内瘤变, 宫颈癌, 树突状细胞, T细胞, 免疫组织化学定量分析, 人

Abstract: Objective To investigate the number, distribution and expression intensity of dendritic cells and T lymphocytes in human cervical intraepithelial neoplasia and invasive carcinoma for seeking biotherapeutic evidence of cervical carcinoma. Methods The number, distribution and expression intensity of CD1a, S100, CD3, CD8 positive cells were determined in cervical intraepithelial neoplasia and invasive carcinoma and human normal cervix tissue as the control, by using immunohistochemical technique and computerized imaging analysis system. Results Compared with human normal cervical tissue, the number and the expression intensity of CD+1a, S100+, CD+3, CD+8 cells in cervical intraepithelial neoplasia significantly increased (P<0.05) and had a positive relation to histological grade(P<0.05); the number and the expression intensity of four antibody positive cells in invasive carcinoma decreased and had a negative relation to the clinical stage(P<0.05).Conclusion The number and the expression intensity of dendritic cells and T lymphocytes changed in the process of cervical carcinogenesis, and the results suggest that there are some changes in local im

Key words: Cervical intraepithelial neoplasia, Cervical carcinoma, Dendritic cell, T lymphocyte, Immunohistochemical quantity analysis, Human