关键词: β-淀粉样肽SUB>1～42/SUB>, 凋亡, 胰岛素样生长因子结合蛋白-3, 视网膜X受体α, 信号转导子和转录激动子, 海马神经元, 免疫细胞荧光, 免疫印迹法, 大鼠

Abstract: Objective To investigate the effects of IGFBP-3, RXRα and STAT-1 on AβSUB>1-42/SUB> induced apoptosis in rat hippocampus neurons. Methods Apoptosis was induced by fibrillar AβSUB>1-42/SUB>. The percentage of neurons apoptosis was evaluated by microscopy after staining with TUNEL/DAPI. IGFBP-3 and RXRα positive neurons were observed by immunofluorescence. The expression of RXRα and STAT-1 protein were detected by Western blotting. Results After treatment with 20μmol/L AβSUB>1-42/SUB> for 24 hours, the apoptotic hippocampus neurons were shown by TUNEL/DAPI assay. The percentage of apoptotic neurons was increased in a time-dependent manner. During the development of apoptosis, both the percentage of IGFBP-3/ RXRα positive neurons and the expression of RXRα protein increased markedly after 3-6hours (EM>P/EM><0.01). However, the expression of STAT-1 protein significantly dec

Key words: β-amyloidSUB>1-42/SUB>, Apoptosis, Insulin-like growth factor-binding protein-3(IGFBP-3), Retinoid X receptor-α(RXRα), STAT-1, Hippocampus neuron, Immunofluorescence, Western blotting, Rat