›› 2008, Vol. 39 ›› Issue (6): 919-922.doi:
• 论著 • 上一篇 下一篇
蒋吉英*; 于树娜 ;齐安东 ;张杰 ;王箐 ;王晓萃; 张皓云; 李洪先
收稿日期:
修回日期:
出版日期:
通讯作者:
Received:
Revised:
Online:
Contact:
关键词: 肝内胆管, 胚胎发育, 肝细胞核因子6, 反转录-聚合酶链反应, 免疫组织化学, 小鼠
Abstract: Objective To investigate the function of hepatocyte nuclear factor 6(HNF 6) during the development of intrahepatic biliary. Methods The expression of HNF6 at different stages of intrahepatic bile ducts embryonic development and in the adult mouse liver were detected by RT-PCR and immunohistochemical staining. Results RT-PCR results showed that the expression of HNF6 was detected in the liver at E9d, the time of liver formation onset. Then, HNF6 disappeared transiently at E13d, but it appeared again at E15d. Its expression lasted till birth. According to the immunohistochemical staining studies, the immunohistochemical positive reaction of CK19, which dispersed in the liver cords, appeared at E13d. At E15d, CK19-positive cells formed a single cell layer called the ductal plate around the branches of the portal vein located close to the hilum. Later, these immunohistochemical positive cells were confined to the ductal plate and biliary epithelial cells. The immunohistochemical positive reaction of HNF6 was detectable in most cells of liver cords at E9-11d, which became undetectable at E13d. Similar to CK19 at E15-17d, the immunohistochemical positive reaction of HNF6 could be observed at P1d. BR>Conclusion HNF6 has no important role in the specification of liver during rat embryonic development. It may play a role in the onset of liver development, the differentiation of hepatic stem cell towards biliary epithelial cells and the maintenance of the morphological characteristic of biliary epithelial cells. BR>
Key words: Intrahepatic biliary, Embryonic development, Hepatocyte nuclear factor 6, RT_PCR, Immunohistochemistry, Mouse
中图分类号:
R329
蒋吉英;于树娜;齐安东;张杰;王箐;王晓萃;张皓云;李洪先. 肝细胞核因子6在小鼠肝内胆管发育过程中的表达[J]. , 2008, 39(6): 919-922.
0 / / 推荐
导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks
链接本文: https://jpxb.bjmu.edu.cn/CN/
https://jpxb.bjmu.edu.cn/CN/Y2008/V39/I6/919
酒精性肝纤维化核因子κB信号通路与性别的差异
三七总皂苷抑制小鼠星形胶质细胞活化缓解完全弗氏佐剂所致炎性痛
抑制lncRNA TUG1下调核苷酸结合寡聚结构域样受体蛋白1炎症小体在延缓阿尔茨海默病进展的作用
创伤性颅脑损伤中神经功能障碍与认知功能损伤的动态变化
脑源性神经营养因子在牦牛与黄牛端脑不同区域的表达与分布
白细胞介素6对脂多糖诱导所致类子痫前期大鼠有核红细胞的影响
利拉鲁肽对百草枯诱导的小鼠帕金森病模型炎症及线粒体融合/分裂的影响
小鼠棕色脂肪透射电子显微镜样品固定方法的改良