关键词: 膜联蛋白A5, 子宫颈鳞癌, 免疫印迹法, 免疫组织化学, 人

Abstract: P>Objective To observe whether the expression of Annexin A5(ANXA5) changes in human uterine cervical squamous cell carcinomas(UCSCC). Methods 25 UCSCC tissues and 15 normal human uterine cervical tissues were collected. Each sample was lysed in lysis buffer.Whole protein of the supernatant was estimated by BCA-100 Protein Quantitative Analysis Kit. The expressions of ANXA5 in UCSCCs and normal uterine cervical tissues were detected respectively with Western blotting. To further ensure the expression of ANXA5 in UCSCCs, another 45 UCSCC specimens and 20 normal cervical tissues were collected. ANXA5 expression was detected by in situ hybridization and immunohistochemistry respectively. Results The staining of ANXA5 band with Western blotting in UCSCCs was much heavier than that in normal uterine cervical tissues and the expression of ANXA5 was found much higher in UCSCCs by immunohistochemistry and in situ hybridization.Conclusion Expression of ANXA5 was up-regulated in human UCSCCs.There’s some relationship between uterine cervical squamous cell carcinoma and ANXA5 p

Key words: Annexina5, Uterine cervical squamous cell carcinoma, Western blotting, Immunohistochemistry, Human