解剖学报

›› 2008, Vol. 39 ›› Issue (5): 636-639.doi:

• 论著 • 上一篇    下一篇

轴突成束和延伸蛋白ζ-1基因在1型和2型星形胶质细胞中的表达

何江虹1,2 ;张晔1 ;夏春林1*; 张万钰2; 朱婷2 ;万明辉1   

  1. 1.苏州大学医学院解剖学教研室暨细胞神经生物学研究室，江苏 苏州 215123; 2.南通大学 江苏省神经再生重点实验室，江苏 南通 226001
  • 收稿日期:2007-06-27 修回日期:2007-08-02 出版日期:2008-10-06
  • 通讯作者: 夏春林

EXPRESSION OF FASCICULATION AND ELONGATION PROTEIN ZETA1 IN TYPE-1 AND TYPE-2 ASTROCYTES

  1. 1.Department of Anatomy and Cytoneurobiology, Medical College of Sochoow University, Jiangsu Suzhou 215123, China；2.Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong University, Jiangsu Nantong 226601, China
  • Received:2007-06-27 Revised:2007-08-02 Online:2008-10-06
  • Contact: XIA Chun-lin

关键词: 轴突成束和延伸蛋白ζ-1, 星形胶质细胞, 胶质细胞谱系, 基因表达谱芯片, 反转录-聚合酶链式反应, 大鼠

Abstract: Objective To observe and analyze the expression of fasciculation and elongation protein zeta-1 (FEZ1) gene in purified type-1 astrocytes (T1A) and type-2 astrocytes (T2A). Methods By the technique of gene-chip, some related genes in the different gene expression profiles of purified T1A and T2A were compared and analyzed. Increased mRNA levels of FEZ1 in T2A was further confirmed by RT-PCR. Results 138 different genes were differentially expressed in T1A and T2A, 60 of which were highly expressed in T1A and 78 in T2A. The expression of FEZ1 was up-regulated in T2A, and the expression of FEZ1 was detected in the different parts (cerebral cortex, olfactory bulb and spinal cord) of the central nervous system of P1 rats.Conclusion FEZ1 gene is among several genes that expressed differently in T1A and T2A. The expression of FEZ1 is found in central nervous system of P1 rats. FEZ1 may play an important role in the development and regeneration of central nervous system.

Key words: Fasciculation and elongation protein zeta-1(FEZ1), Astrocyte, Glial lineage, Gene chip, RT-PCR, Rat

中图分类号: