解剖学报

›› 2009, Vol. 40 ›› Issue (5): 691-695.doi: 10.3969/j.issn.0529-1356.2009.05.001

• 论著 •    下一篇

小RNA干扰Akt2对U87恶性胶质瘤细胞迁移和侵袭能力的影响

张宝刚1,4; 谷峰2 ;李文良3; 郭华1;张宁1;马勇杰1*   

  1. 1.天津医科大学附属肿瘤医院研究所中心实验室;2.乳腺病理研究室;3.颅脑肿瘤科，天津 300060; 4.潍坊医学院乳腺病理研究室，潍坊 261053
  • 收稿日期:2008-12-08 修回日期:2009-01-16 出版日期:2009-10-06
  • 通讯作者: 马勇杰

EM>In vitro/EM> study of Akt2 function on U87 malignant glioma cells migration and invasion

  1. 1.Department of Core Laboratory, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060,China;2.Department of Breast Pathology and Research Laboratory, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060,China;3.Department of Neurosurgery and Neuro-Oncology，Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060,China;4.Department of Breast Pathology, Weifang Medical University, Weifang 261053,China
  • Received:2008-12-08 Revised:2009-01-16 Online:2009-10-06
  • Contact: MA Yong-jie

关键词: 胶质瘤, 细胞运动, 侵袭, 小RNA干扰, 免疫印迹法

Abstract: Objective To detect the effect of Akt2 on U87 glioblastoma cells migration and invasion by Akt2 small RNA interference technology EM>in vitro/EM>.Methods Akt2 expression was knocked down in U87 glioblastoma cells by Akt2 siRNA, stable clones were used to perform wound healing assay, F-actin polymerization assay, adhesion assay and matrigel invasion assay. Western blotting was used to check the expression level of phosphorylated cofilin and LIMK. Results Result of wound healing assay suggested that motility of U87 cells was significantly decreased with deficiency of Akt2; U87 cells with Akt2 deficiency was not capable of assembling actin effectively compared to control cells by F-actin polymerization assay. Further, phosphorylated cofilin and LIMK expression which are important for cellular motility were all decreased with Akt2 deficiency. Besides, adhesion and invasion ability of U87 cells were significantly impaired after Akt2 was knocked down. Conclusion Akt2 is important for U87 cells migration and invasion. Akt2 deficiency will lead to impairment of U87 glioblastoma cells motility, adhesive and invasive ability EM>in vitro/EM>.

Key words: Glioma, Cell migration, Invasion, Small interference RNA, Western blotting

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