解剖学报

›› 2009, Vol. 40 ›› Issue (2): 340-343.doi: 10.3969/j.issn.0529-1356.2009.02.035

• 技术方法 • 上一篇    

利用亮甲酚蓝染色筛选优质猪卵母细胞

田江天1;王振坤1 ;郑重1;孙爽1;徐萌2;刘忠华1*   

  1. 1.东北农业大学生命科学学院，哈尔滨 150030;2.浙江大学材料与化学工程学院材料科学与工程学系，杭州 310027
  • 收稿日期:2007-12-19 修回日期:2008-04-08 出版日期:2009-04-06
  • 通讯作者: 刘忠华

Selection of developmentally competent pig oocytes using brilliant cresyl blue staining

  1. 1.College of Life Science, Northeast Agricultural University, Haerbin 150030, China;2.Department of Materials Science and Engineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China
  • Received:2007-12-19 Revised:2008-04-08 Online:2009-04-06
  • Contact: LIU Zhong-hua

关键词: 卵母细胞, 核移植, 亮甲酚蓝（BCB）染色, 猪

Abstract: P>Objective To enhance efficiency of EM>in vitro/EM> embryos culture system of pig using brilliant cresyl blue (BCB) stain. Methods To stain the cumulusoocyte complexes through BCB then evaluate oocytes maturation rates and developmental competence of early embryos. Results The percentage (91.6%) of the MⅡ stage oocytes of pigs was higher in the BCBcolorable group (BCBSUP>+/SUP> ) than those (64.0%) in the noncolorable (BCBSUP>－/SUP>) and control (77.5%) groups (EM>P/EM><0.05). The pig parthenogenetic blastocyst rate (34.9%) was significantly higher in the BCB+ group than those in the control and BCBSUP>－/SUP> groups, (23% and 9.5%) respectively (EM>P/EM><0.05). The rate (23.9%) of pig somatic cell nucleus-transferred embryos into blastocyst was significantly higher in the BCBSUP>+/SUP> group than those in the control and the BCB

Key words: Oocyte, Nuclear transfer, Brilliant cresyl blue （BCB）staining, Pig

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