解剖学报

解剖学报 ›› 2016, Vol. 47 ›› Issue (4): 510-515.doi: 10.16098/j.issn.0529-1356.2016.04.013

• 组织学胚胎学发育生物学 • 上一篇    下一篇

α1,3半乳糖转移酶基因敲除巴马克隆猪与核供体巴马猪心、肺解剖学与组织学的比较

韩晓娜1 曾国敏2 杨阳1 潘登科3* 杜晓华1*   

  1. 1. 甘肃农业大学动物医学院，兰州 730070; 2. 甘肃农业大学生命科学技术学院，兰州 730070;3. 中国农业科学院北京畜牧兽医研究所，农业部畜禽遗传资源与种质创新重点实验室，北京 100193

  • 收稿日期:2015-12-15 修回日期:2016-02-05 出版日期:2016-08-06 发布日期:2016-08-06
  • 通讯作者: 潘登科;杜晓华 E-mail:duxh@gsau.edu.cn
  • 基金资助:

    国家重点基础研究发展计划

Anatomical and histological comparisons on heart and lung between α1,3-galactosyltransferase gene-deficient cloned and noncloned Bama miniature pig

HAN Xiao-na1 ZENG Guo-min2 YANG Yang1 PAN Deng-ke 3* DU Xiao-hua 1*   

  1. 1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2. College of Life Science and Technology，Gansu Agricultural University，Lanzhou 730070，China; 3. Key Laboratory of Farm Animal Genetic Resource and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

  • Received:2015-12-15 Revised:2016-02-05 Online:2016-08-06 Published:2016-08-06
  • Contact: PAN Deng-ke ; DU Xiao-hua E-mail:duxh@gsau.edu.cn

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摘要：

目的 探讨α1,3半乳糖转移酶（GGTA1）基因敲除巴马克隆猪与核供体巴马猪心、肺解剖学与组织学发育变化的异同。 方法 选取GGTA1基因敲除巴马克隆猪2头(BM164和BM155)及核供体巴马猪1头(BMM2)，利用大体解剖学和常规石蜡切片HE染色技术，对其心脏和肺脏进行解剖学与组织学比较研究。 结果 核供体成年健康巴马猪心脏呈倒置圆锥形，前缘凸，后缘短而直，心表面冠状沟和左右纵沟清晰可见；心壁结构完整，可分3层，心内膜较薄，心室内膜下有较多浦肯耶纤维，心肌排列紧密，横纹清晰，闰盘少且不清楚，心外膜较厚，无髓神经纤维明显。GGTA1基因敲除巴马克隆猪与核供体巴马猪相比较，心脏在解剖学与组织学构造上均未见明显差异。核供体成年健康巴马猪肺脏呈粉红色海绵状，质软而轻，富有弹性；肺脏小支气管软骨丰富，黏膜下层内气管腺明显，可见炎性细胞，细支气管黏膜皱褶呈指状向管腔凸起，周围有炎性细胞浸润，肺泡大小较一致，肺泡壁较厚，尘细胞少见。GGTA1基因敲除巴马克隆猪肺脏解剖学与组织学结构与核供体巴马猪基本一致。 结论 GGTA1基因敲除巴马克隆猪与核供体巴马猪心、肺解剖学构造无明显差异，组织学结构正常，心、肺发育良好。

关键词: α1,3半乳糖转移酶基因, 基因敲除, 克隆, 心脏, 肺脏, 大体解剖学, HE染色, 巴马猪

Abstract:

Objective To study anatomical and histological differences on hearts and lungs between α1,3-galactosyltransferase(GGTA1)gene-deficient cloned pigs and noncloned Bama miniature pig(as donor cell for somatic cell nuclear transfer). Methods Anatomical and histological structures on hearts and lungs of two α1,3-galactosyltransferase gene-deficient cloned pigs (BM164，BM155) and one healthy noncloned Bama miniature pig(BMM2) were observed by using gross anatomy and HE staining. Results The heart of noncloned Bama pig was inverted conical, anterior border convexity and posterior border short and straight, obvious coronary sulcus, sulcus longitudinalis sinister and sulcus longitudinalis dexter. The cardiac wall was divided into three layers. The endocardium was thin and there were some Purkinje fibers in the ventricle. The cardiac muscle fibers arranged densely, clear cross striation and fewer intercalated disk in the cardiac muscle. The epicardium was thick, and had obvious unmyelinated nerve fibers. Compared with noncloned Bama pig, the hearts of GGTA1 gene-deficient cloned pigs showed no significant anatomical and histological differences. The lung of noncloned Bama miniature pig was pink spongy, soft and light. Histological observation showed that there were some cartilages around small bronchi, some tracheal glands and inflammatory cells in submucosa, few dust cells and thicker alveolar wall. Bronchial mucosal folds were finger-like projections into the lumen, which was infiltrated by some inflammatory cells. The structures of anatomical and histological structures in lungs between α1,3-galactosyltransferase gene-deficient cloned pigs and noncloned Bama pig were basically same. Conclusion The hearts and lungs of GGTA1 gene-deficient cloned pigs and noncloned Bama miniature pig showed no significant anatomical and histological differences.

Key words: α1,3-galactosyltransferase, Gene knockout, Clone, Heart, Lung, Gross anatomy, HE staining, Bama miniature pig