解剖学报

›› 2009, Vol. 40 ›› Issue (5): 755-759.doi: 10.3969/j.issn.0529-1356.2009.05.012

• 论著 • 上一篇    下一篇

孕酮调节小鼠脾脏CD11c阳性树突状细胞白细胞介素和TNF-α的表达

杨林松1*; 冯敏敏1 ;周正球2; 侯亚义3   

  1. 1.江苏工业学院化学化工学院生物工程实验室，常州 213164; 2. 常州市中医院风湿科，常州 213000;3.南京大学医学院免疫与生殖生物学实验室，南京 210093
  • 收稿日期:2008-07-14 修回日期:2008-09-23 出版日期:2009-10-06
  • 通讯作者: 杨林松

Regulation of progesterone on interleukins and TNF-α expression ofmurine CD11c positive splenic dendritic cells

  1. 1. Biological Engineering Laboratory, Faculty of Chemistry and Chemical Engineering, Jiangsu Polytechnic University, Changzhou 213164, China; 2. Department of Rheumatology, Changzhou Chinese Medicine Hospital, Changzhou 213000, China; 3. Immunology and Reproductive Biology Laboratory, Medical School，Nanjing University, Nanjing 210093, China
  • Received:2008-07-14 Revised:2008-09-23 Online:2009-10-06
  • Contact: YANG Lin-song1

关键词: 孕酮, 脾脏树突状细胞, 细胞因子, PT-PCR, 小鼠

Abstract: Objective To elucidate the effect of progesterone (Prog) on the cytokine expressions of murine CD11c positive splenic dendritic cells (SDCs). Methods SDCs were purified by immune beads and treated with various concentrations of Prog for 24hours, then cytokine expressions of SDCs in the mRNA level were analyzed through reverse transcription-polymerase chain reaction (RT-PCR). Results Prog increased the transcript of IL-6 in middle dose group but decreased it in high dose, and the level of IL-10 was increased in low and middle dose groups while decreased in high dose group. Furthermore, the transcripts of IL-12 and TNF-α were increased and decreased respectively in all Prog treated groups.Conclusion Prog might regulate organ immune response through influencing cytokine expressions of SDCs. Low- and middle-dose Prog switch organ immune response to Th2-type, while high-dose Prog develops a Th1-type immune response.

Key words: Progesterone, Splenic dendritic cell, Cytokine, RT-PCR, Mouse

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