解剖学报

›› 2009, Vol. 40 ›› Issue (5): 807-810.doi: 10.3969/j.issn.0529-1356.2009.05.023

• 论著 • 上一篇    下一篇

17β-雌二醇对大鼠心肌缺血再灌注损伤的影响

彭兴春1,2;田宗文2*;平灵艳2;施俊2;王乔2   

  1. 1.郧阳医学院解剖学教研室，湖北十堰 442000;2.武汉大学基础医学院人体解剖与组织胚胎学系，武汉 430071
  • 收稿日期:2008-01-12 修回日期:2008-05-30 出版日期:2009-10-06
  • 通讯作者: 田宗文

Effect of 17β-estradiol on myocardial ischemia-reperfusion injury of rats

  1. 1. Department of Human Anatomy, Yunyang Medical College, Hubei Shiyan 442000, China;2. Department of Anatomy and Histology-Embryology, Wuhan University School of Medicine, Wuhan 430071, Chin
  • Received:2008-01-12 Revised:2008-05-30 Online:2009-10-06
  • Contact: TIAN Zong-wen

关键词: 17β-雌二醇, 缺血再灌注, c-fos, 流式细胞术, 免疫组织化学, 大鼠

Abstract: Objective To explore effects of 17β-estradiol preconditioning on myocardial ischemia-reperfusion(I/R) injury of rats and its mechanism. Methods Totally 30 female SD rats were ovariectomized and divided randomly into C group (sham), I/R group and I/R+ESUB>2 /SUB>group (17β-estradiol pretreatment). After 2 weeks of pretreatment with 17β-estradiol, all rats were subjected to the left anterior descending coronary occlusion for 30 minutes and reperfusion for 2 hours. The sizes of myocardial infarction area were measured by triphenyltetrazolium chloride (TTC) staining; the apoptotic rate was assayed by flow cytometer, the protein expression of EM>c-fos/EM> proto-oncogene in cardiomyocytes was detected by semiquantitative immunohistochemical technique. Results 17β-estradiol significantly attenuated the infarct size of myocardium injured by ischemia-reperfusion, decreased the apoptotic rate in cardiomyocytes, also inhibited the expression of c-Fos protein in cardiomyocytes.Conclusion These results suggest that estrogen protects the heart against ischemia-reperfusion injury of rat，its cardioprotective mechanism

Key words: 17β-Estradiol, Ischemia/reperfusion, c-fos, Flow cytometry, Immunohistochemistry, Rat

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