›› 2010, Vol. 41 ›› Issue (1): 75-79.doi: 10.3969/j.issn.0529-1356.2010.01.015

• 论著 • 上一篇    下一篇

NF-κB及调节活化正常T细胞表达和趋化因子在升主动脉瘤形成中的表达

郭万伟;柏树令*;王军;范军;田晓红   

  1. 中国医科大学基础医学院人体解剖学教研室,沈阳 110001
  • 收稿日期:2009-04-03 修回日期:2009-07-13 出版日期:2010-02-06
  • 通讯作者: 柏树令

Expression of NF-κB and regulated upon activation normal T cell expressed and secreted chemokine in experimental abscending aortic aneurysm rat model

  1. Department of Human Anatomy,China Medical University,Shenyang11001,China
  • Received:2009-04-03 Revised:2009-07-13 Online:2010-02-06
  • Contact: BAI Shu-ling

关键词: NF-κB, 调节活化正常T细胞表达和趋化因子, 升主动脉瘤, 反转录-聚合酶链反应, 免疫组织化学, 大鼠

Abstract: Objective To investigate the expression of nuclear factor kappaB(NF-κB) and regulated upon activation normal T cell expressed and secreted chemokine(RANTES) during the formatiom of ascending aortic aneurysm. Methods Forty Wistar rats were randomly divided into the control group(n=20) and the experimental group(n=20).The rat models were made by ligating the ascending aorta. The ascending aortas were taken after ligation for 3months. Immunohistochemistry staining was performed to detect the protein expression of NF-κB and RANTES. The expression of NF-κB and RANTES mRNA were detected by RT-PCR. Results Immunohistochemisry staining results showed NF-κB and RANTES expression significantly increased in aneurysm, while there was a little positive staining in the control group. RT-PCR results indicated that the expression levels of NF-κB and RANTES in the aneurysm were stonger than that of the control group. The expression of NF-κB and RANTES mRNA were remarkably correlated. Conclusion The expression of NF-κB and RANTES in ascendin aortic aneurysm are stronger than that in

Key words: NF-κB, RANTES, Ascending aortic aneurysm, RT-PCR, Immunohistochemistry, Rat

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