›› 2010, Vol. 41 ›› Issue (1): 124-127.doi: 10.3969/j.issn.0529-1356.2010.01.024
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王中伟;岳顺利;朱佳伟;曹效彰;孙琪;王媛;周佳勃*
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关键词: 胚胎极性, Em-Ab轴, 卵裂球, 显微注射, 小鼠
Abstract: Objective Kunming strain(KM) mice were used as animal models. Nontoxic dextran conjugated with tetramethylrhodamine（TMR）and fluorecein isothiocyante（FITC）was microinjected to two of the 2-cell blastomere as molecular probe to trace the development fate of the blastomere ,in order to figure out the mechanisms of the formation of Em-Ab axis. Methods FITC- dextran was injected to zygote in order to make sure if it is noxious. Two blastomeres of 2-cell embryo were injected FITC- dextran and TMR- dextran respectively. Results When labeled embryo develeped to blastocyst, distribution of progeny of 2-cell embryo blastomeres can be detected.Conclusion The cells of blastomere randomly distributed either embryonic parts or extra
Key words: Embryo polarization, Em-Ab axes, Blastomere, Microinjection, Mouse
中图分类号:
Q132.8
王中伟;岳顺利;朱佳伟;曹效彰;孙琪;王媛;周佳勃. 小鼠2-细胞胚胎卵裂球后代在囊胚中随机分布[J]. , 2010, 41(1): 124-127.
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链接本文: https://jpxb.bjmu.edu.cn/CN/10.3969/j.issn.0529-1356.2010.01.024
https://jpxb.bjmu.edu.cn/CN/Y2010/V41/I1/124
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