›› 2010, Vol. 41 ›› Issue (1): 124-127.doi: 10.3969/j.issn.0529-1356.2010.01.024

• 论著 • 上一篇    下一篇

小鼠2-细胞胚胎卵裂球后代在囊胚中随机分布

王中伟;岳顺利;朱佳伟;曹效彰;孙琪;王媛;周佳勃*   

  1. 东北农业大学生命科学学院动物组织学与胚胎学教研室, 哈尔滨 150030
  • 收稿日期:2008-08-22 修回日期:2008-10-06 出版日期:2010-02-06
  • 通讯作者: 周佳勃

Progeny of 2-cell embryo blastomeres distribute in mouse blastocyst randomly

  1. P>College of Life Science, Northeast Agricultural University, Harbin150030, China/P>
  • Received:2008-08-22 Revised:2008-10-06 Online:2010-02-06
  • Contact: ZHOU Jia-bo

关键词: 胚胎极性, Em-Ab轴, 卵裂球, 显微注射, 小鼠

Abstract: Objective Kunming strain(KM) mice were used as animal models. Nontoxic dextran conjugated with tetramethylrhodamine(TMR)and fluorecein isothiocyante(FITC)was microinjected to two of the 2-cell blastomere as molecular probe to trace the development fate of the blastomere ,in order to figure out the mechanisms of the formation of Em-Ab axis. Methods FITC- dextran was injected to zygote in order to make sure if it is noxious. Two blastomeres of 2-cell embryo were injected FITC- dextran and TMR- dextran respectively. Results When labeled embryo develeped to blastocyst, distribution of progeny of 2-cell embryo blastomeres can be detected.Conclusion The cells of blastomere randomly distributed either embryonic parts or extra

Key words: Embryo polarization, Em-Ab axes, Blastomere, Microinjection, Mouse

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