解剖学报

›› 2010, Vol. 41 ›› Issue (1): 132-136.doi: 10.3969/j.issn.0529-1356.2010.01.026

• 论著 • 上一篇    下一篇

p16INK4A在动情周期和早孕期小鼠子宫内膜的表达

郗强1;朱兴春2;姜蓉3;杨戎1* ;魏莎莉1;张文文1   

  1. 1.重庆医科大学生殖生理学教研室，重庆400016; 2.川北医学院附属医院检验科，四川 南充637000；3.重庆医科大学干细胞与组织工程学研究室，重庆400016
  • 收稿日期:2009-02-14 修回日期:2009-05-11 出版日期:2010-02-06
  • 通讯作者: 杨戎

Expression of p16SUP>INK4A /SUP>in mouse endometrium during the estrus cycle and the early pregnancy period

  1. 1.Department of the Reproductive Physiology,Chongqing Medical University,Chongqing400016,China;2. Department of Clinical Laboratory,Affiliated Hospital of North Sichuan Medical College, Sichuan Nanchong63700,China;3. Laboratory of Stem Cell and Tissue Engineering,Chongqing Medical University,Chongqing400016,China
  • Received:2009-02-14 Revised:2009-05-11 Online:2010-02-06
  • Contact: YANG Rong

关键词: p16SUP>INK4A/SUP>, 子宫内膜, 反转录-聚合酶链式反应, 免疫组织化学, 免疫印迹法, 小鼠

Abstract: Objective To investigate the expression of tumor suppressor gene p16SUP>INK4A/SUP> in mouse endometrium during the estrus cycle and early pregnancy and its possible role in blastocyst implantation. Methods Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of p16INK4A mRNA,immunohistochemistry and Western blotting were applied to detect p16SUP>INK4A/SUP> protein in mouse endometrium tissues during the estrus cycle and early pregnancy.　 Results The intensity of p16SUP>INK4A/SUP> mRNA expression in mouse early pregnancy was higher than that in the estrus cycle.Compared with the other 3 stages, the level of p16SUP>INK4A/SUP> mRNA expression at estrus was obviously higher. During the early pregnancy, the level of p16SUP>INK4A/SUP> mRNA expression increased steadily from day 2 to day 5,reaching the maximal level on day 5,then decreasing. Both immunohistochemical and Western blotting analysis showed the same results in expression patterns of p16SUP>INK4A/SUP> protein for mouse endometrium tissues as those results of RT-PCR.Conclusion p16SUP>INK4A/SUP> is involved in the embryos penetrating into the endometrial barrier.

Key words: p16SUP>INK4A/SUP>, Endometrium, RT- PCR, Immunohistochemistry, Western blotting, Mouse

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