›› 2010, Vol. 41 ›› Issue (2): 191-196.doi: 10.3969/j.issn.0529.1356.2010.02.005

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P> 三七皂苷Rg1上调SD大鼠大脑皮质巯醇抗氧化物(酶)发挥抗衰老作用/P>

王婷婷1; 李兴国2;李守民2; 傅希玥2;王文敏1 ;孙俊2; 艾青龙1*; 陆地2*    

  1. 1.昆明医学院附属一院神经内科,昆明 650032; 2.昆明医学院人体解剖学教研室,昆明 650031
  • 收稿日期:2009-06-22 修回日期:2009-07-23 出版日期:2010-04-06
  • 通讯作者: 艾青龙; 陆地

Notogisenoside Rg1 upregulates the thiol antioxidants and resists aging in rats

  1. 1.Department of Neurology, the First Affiliated Hospital of Kunming Medical University, Kunming 650032,China;2.Department of Anatomy, Kunming Medical University, Kunming 650031, China
  • Received:2009-06-22 Revised:2009-07-23 Online:2010-04-06
  • Contact: AI Qing-long;LU Di

关键词: 三七皂苷Rg1, β淀粉样蛋白1-42, D-半乳糖, 巯醇抗氧化物(酶), Caspase-3前体蛋白, 免疫组织化学, 免疫印迹法, 大鼠

Abstract: Objective In order to investigate anti-ageing mechanisms of the notoginsenoside Rg1,we used AβSUB>1-42/SUB> and D-galactose to establish aging rat model. Methods Ninety rats were divided into three groups at random: sham group, model group, treatment group. Aging rat models were established by injecting peritoneally Dgalactose (100 mg/kg) to the rats for 56 days and after 35 days aggregated AβSUB>1-42/SUB>(μg) was injected to the right lateral ventricle of rats. Meantime, rats were treated by intragastric administration the notoginsenoside Rg1 Then spatial memory of experimental rats was examined with the Morris water maze(MWM). The thiol antioxidants including glutathione reductase (GR) and glutathione peroxidase (GSH-Px) activities were examined by colorimetric method. The concentration of the proCaspase-3 and Bcl-2 were examined by the immunohistochemistry and Western blotting method. Results In aging model rats escape latercies were significantly prolonged (P<0.05), while decreases were seen in the time of staying the third quadrants of platform, the number of crossing over a platform, the concentration of the GR, GSH-Px, and proCaspase-3 as compared with the sham group(P<0.05). After treatment of the notoginsenoside Rg1, the aging model rats exhibited significant increases in the time of staying the third quadrants of platform, the number of crossing over a platform, the concentration of the GR, GSH-Px, and proCaspase-3(P<0.05), while a decrease was observed in escape latercies as compared to control group(P<0.05). Moreover there was no significant difference in the expression of the Bcl-2(P>005). Conclusion The results from our study indicate that the notoginsenoside Rg1 could improve the oriented

Key words: Notoginsenoside Rg1, AβSUB>1-42/SUB>, D-galactose, Thiol antioxidant, Pro-Caspase-3, Immunohistochemistry, Western blotting, Rat

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