解剖学报

›› 2010, Vol. 41 ›› Issue (2): 180-184.doi: 10.3969/j.issn.0529.1356.2010.02.003

• 论著 • 上一篇    下一篇

睾酮对小鼠坐骨神经损伤后脊髓前角运动神经元的保护作用

张燕红1 ;张星漪1; 鞠躬1,2*    

  1. 1.上海交通大学生命科学技术学院神经科学研究所，上海 200240;2. 第四军医大学全军神经科学研究所，西安 710032
  • 收稿日期:2009-09-07 修回日期:2009-11-12 出版日期:2010-04-06
  • 通讯作者: 鞠躬

Protection of testosterone on spinal cord anterior horn motor neurons after sciatic nerve injury in mice

  1. 1.Institute of Neuroscience, School of Life Sciences & Biotechnology, Shanghai Jiaotong University, Shanghai 200240,China;2.Institute of Neuroscience, the Fourth Military Medical University, Xi′an 710032,China
  • Received:2009-09-07 Revised:2009-11-12 Online:2010-04-06
  • Contact: JU Gong

关键词: 睾酮, 运动神经元, 坐骨神经, 尼氏染色, 小鼠

Abstract: Objective To investigate the protective effect of testosterone on motor neuron after sciatic nerve injury. Methods Twelve adult C57 male mice were randomly divided into two groups: the sesame oil control group (n= 6) and the testosterone experimental group (n= 6). Unilateral sciatic nerve cutting severed as the animal model. Following operation, sesame oil or testosterone was administered for the control and experimental animals respectively via subcutaneous injection every other day for two weeks. The number and soma area of the anterior horn motor neurons in the lumbosacral cord of the sciatic nerve injury side were then measured on Nissl stained sections. Results In the testosterone group the motor neurons appeared healthier than that in the sesame control group. The neurons were plumper and showed more processes. Their number and average soma size were significantly larger than that in the sesame control (P<0.01). Conclusion The findings demonstrate that testosterone has a significant protective effect on sciatic nerve motor neurons after nerve injury.

Key words: Testosterone, Motoneuron, Sciatic nerve, Nissl staining, Mouse

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