解剖学报

›› 2010, Vol. 41 ›› Issue (2): 224-227.doi: 10.3969/j.issn.0529-1356.2010.02.011

• 论著 • 上一篇    下一篇

脂多糖对小鼠铁代谢相关基因mRNA水平表达的影响

郭晓强1,2; 马亚娟1 ;田莎1 ;丁卉1 ;任国庆3 ;常彦忠1 ;段相林1*   

  1. 1.河北师范大学生命科学学院;河北省动物生理生化与分子生物学重点实验室，石家庄 050016; 2.解放军白求恩军医学院生物化学教研室，石家庄 050081; 3.解放军260医院检验科，石家庄 050001
  • 收稿日期:2009-04-09 修回日期:2009-04-29 出版日期:2010-04-06
  • 通讯作者: 段相林

Effects of lipopolysaccharide on mice iron metabolism related genes mRNA expression

  1. 1.College of Life Science, Hebei Normal University, the Key Laboratory of Animal Physiology,Biochemistry and Molecular Biology of Hebei Province; Shijiazhuang 050016,China;2.Department of Biochemistry, Bethune Military Medical College, Shijiazhuang 050081,China; 3. Clinical Laboratory the 260th Hospital of PLA, Shijiazhuang 050041,China
  • Received:2009-04-09 Revised:2009-04-29 Online:2010-04-06
  • Contact: DUAN Xiang-lin

关键词: 脂多糖, 白细胞介素-6, 铁调素, 膜铁转运蛋白1, 转铁蛋白受体1, 反转录-聚合酶链式反应, 小鼠

Abstract: Objective To investigate the effects of lipopolysaccharide (LPS) on mRNA expression of iron metabolism related genes. Methods Ten male mice （2 months） were injected intraperitoneally with lipopolysaccharide（0.5 μg/g） After 6 hours, mice were sacrificed and then sera, liver and spleen were collected. The mice blood routine was measured. The serum iron and total iron binding capacity (TIBC) were determined with reagent kit. The quasi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was performed for mRNA of hepatic hepcidin(HP), ferroportin1(Fpn1), transferrin receptor 1(TfR1) and spleenic HP, Fpn1 and interleukin6(IL-6). Results The serum iron and TIBC were reduced in mice injected LPS, which exhibited mild anemia(EM>P/EM> 0.5). LPS can increase the expression of hepatic hepcidin and decrease Fpn1 and TfR1 in liver after LPS administration 6 hours(EM>P /EM>0.05). In spleen, IL-6 was upregulated and Fpn1 downr

Key words: Lipopolysaccharide, Interleukin-6, Hepcidin, Ferroportin 1, Transferrin receptor 1, RT-PCR, Rat

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