关键词: 阿尔茨海默病, 发育, 谷氨酸受体, γ-氨基丁酸受体, 免疫荧光, 小鼠

Abstract: Objective In order to understand the relationship between the receptors of excitatory and inhibitory neurotransmitter and Alzheimer disease(AD), the expression of glutamate receptors and GABASUB>Aα1-/SUB>SUB>6 /SUB>in the CA3 region of hippocampus of APPswe transgenic mice was investigated. Methods These mice from postnatal day 0 to postnatal day 360 were used in the study with the N-methyl-D-asprtate receptor 1(NMDAR1), GluR2/3 and GABASUB>Aα1-6/SUB> immunofluorescent stainings. In the meantime, Western blotting was carried out to detect the expressions of NMDAR1 and GABASUB>Aα1-6/SUB> in the hippocampus as well. Results The numbers of NMDAR1,GluR2/3 and GABASUB>Aα1-6 /SUB> positive neurons increased obviously after P7, in both wild type and transgenic mice, with the peaks at P30 However, in aged animals(P360), NMDAR1 and GluR2/3 lost in transgenic mice with statistically significant difference ( P< 005), comparing with wild type, but the alterations of GABASUB>Aα1-6/SUB> receptor was not obvious (EM>P/EM>>0.05) between wild type and transgenic mice. Conclusion The study showed that glutamate receptors in hippocampu

Key words: Alzheimer disease, Development, Glutamate receptor, GABA receptor, Immunofluorescence, Mouse