›› 2010, Vol. 41 ›› Issue (03): 374-378.doi: 10.3969/j.issn.0529-1356.2010.03.009

• 论著 • 上一篇    下一篇

成人鼻腔嗅区黏膜细胞的分离培养与免疫学特点

王洪美1,2; 任玉水1 ;田国忠1 ;姜晓荣1 ;曹敬丽1 ;刘婧媛1 ;周长满3 ;黄红云1,2*   

  1. 1.北京市虹天济神经科学研究院临床细胞中心,北京 100144;2.北京康复中心神经外科,北京 100144;3.北京大学医学部人体解剖与组织胚胎学系, 北京 100191
  • 收稿日期:2009-09-14 修回日期:2009-10-26 出版日期:2010-06-06
  • 通讯作者: 黄红云

Isolationculture and characteristics of human olfactory mucosa cells

  1. 1.Clinical Cell Center, Beijing Hongtianji Neuroscience Academy, Beijing 100144, China;2.Department of Neurosurgery, Beijing Rehabilitation Center, Beijing 100144,China;3.Department of Anatomy,Histology and Embryology, Peking University Health Science Cente,Beijing 100191,China
  • Received:2009-09-14 Revised:2009-10-26 Online:2010-06-06
  • Contact: HUANG Hong-yun

关键词: 嗅区黏膜, 嗅鞘细胞, 细胞培养, p75, S100, 免疫荧光化学, 细胞移植,

Abstract: Objective To establish a technical method to isolate and culture human olfactory mucosa cells EM>in vitro/EM>, and to study the immunological characteristics of olfactory ensheathing cells(OECs). Methods In this study, seven adult olfactory mucosae were obtained from nasal cavity surgery.Cells were acquired by dissection of olfactory mucosa and purified according to the different attachment time among the various cell types. The cells were observed for morphological characteristics under inverted microscope and identified using immunofluorescence staining with p75,S100 and glial fibrillary acidic protein(GFAP). The characteristics of olfactory ensheathing cells was observed under confocal microscope. Results S100 and p75 positive cells were more common in the olfactory mucosa cells under confocal microscope, GFAP positive cells were relatively rare. In general, S100 and p75 were co-expressed in the same cells, but not the GFAP and S100, p75 expressions. The p75 positive cells presented two completely different morphology. The percentage of S100 and p75 double labeled cells was (35.0±8.3)% under confocal microscope. Conclusion The olfactory ensheathing cells can be isolated from human olfactory mucosa,they are accessible sources for autologous grafting to repair nervous sys

Key words: Olfactory mucosa, Olfactory ensheathing cells, Cell culture, p75, S100, Immunofluorescence, Cell transplantation, Human

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