关键词: 表没食子儿茶素-3-没食子酸酯, 基质金属蛋白酶-1, 愈合, 皮肤, 反转录-聚合酶链式反应, 酶联免疫吸附法, 小鼠

Abstract: Objective To explore the effects of epigallocatechin-3-gallate(EGCG) (50 mg/L) on the expression of MMP-1 mRNA and the levels of MMP-1 during mouse cutaneous wound healing. Methods Fifty-seven mice were divided into control, 50 mg/L EGCG and vehicle groups. The wound skins were prepared at 6, 12, 24, 72, 120 and 168 hours after injury, respectively. The expression of MMP-1 mRNA and levels of MMP-1 in wound healing skins were determined with reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Results It was found that 50 mg/L EGCG inhibits the expression of MMP-1 mRNA and decreases the levels of MMP-1 of the wound tissue at the late stage of wound healing (72 hours after skin injury). Conclusion 50 mg/L EGCG could inhibit the expression of MMP-1 mRNA and the MMP-1 production during cutaneous wound healing, a

Key words: Epigallocatechin-3-gallate (EGCG), Matrix metalloproteinase-1 (MMP-1), Healing, Skin, RT-PCR, ELISA, Mouse